核苷类抗生素丰加霉素(Toyocamycin)可有效防治多种植物真菌病害，已成为农业植物病害防治领域中备受关注的新型抗生素品种。因此，作为目前唯一生物合成丰加霉素的菌株S. diastatochromogenes 1628具有重要的研究价值，但其产量较低，难以满足工业化生产需要，限制了其广泛应用。因此，从分子水平上阐明丰加霉素合成途径及其调控机制具有重要的理论和现实意义。本研究以S. diastatochromogenes 1628为研究对象，克隆生物合成丰加霉素基因簇，利用PCR-targeting技术和中间代谢产物检测等技术方法研究丰加霉素基因簇的生物学功能，阐明其生物合成丰加霉素的分子机制；进一步研究S. diastatochromogenes 1628中全局性调控因子adpAsd对丰加霉素合成基因簇的转录水平影响和作用方式，解析其对生物合成丰加霉素的调控机制。

Toyocamycin (TM) is a member of the nucleoside antibiotic family. Because of similarity to the structure of nucleoside, TM exhibits various biological activities. Recently the TM was found to be highly efficient against a broad range of plant pathogenic fungi, including Rhizoctonia solani Kühn, Fusarium oxysporum f. sp. cucumerinum, F.oxysporum f. sp niveum and Colletotrichum lindemuthianum. Therefore, TM has been recognized as an ideal fungicide that can be utilized to control the occurrence of plant diseases in the agriculture field. Streptomyces diastatochromogenes 1628, which was originally isolated from soil sample, is a producer of TM. So far, the TM production of S. diastatochromogenes 1628 is only report of TM biosynthesis. As a result, S. diastatochromogenes 1628 has the important research value, however, the TM production is limited in this strain, it can’t meet the requirement of industrial production and limited its application. Therefore, analysis of biosynthetic pathway and regulatory mechanism of TM in S. diastatochromogenes 1628 is of theoretical and practical significance to further construct the higher-producer of TM by using metabolic engineering. In this research, the biological functions of the cloned TM biosynthesis gene cluster were investigated by PCR-targeting and detection of intermediates in order to elucidate the biosynthetic pathway of TM at the molecular level in S. diastatochromogenes 1628. Furthermore, on this basis, the effects of central pleiotropic transcriptional activator adpAsd on the transcriptional level of TM biosynthesis gene cluster and mode of its action were determined to reveal the regulatory mechanism involved in TM biosynthesis of S. diastatochromogenes 1628.