切除和杀灭肿瘤细胞是肿瘤治疗的传统思路，效果有限；重编程肿瘤细胞旨在改造肿瘤细胞的恶性发育命运，切中要害；研发重编程肿瘤细胞的活性分子，意义重大。申请人在前期工作中首次发现：牛卵母细胞抽提物能对人肺癌H460细胞进行表观遗传重编程，抽提物中进入到H460细胞内的piRNA在此过程中发挥了重要作用。这提示：牛卵母细胞抽提物的piRNA中，蕴藏着新型抗肿瘤活性分子，值得深入研究。本项目拟将牛卵母细胞抽提物中能进入H460细胞的piRNA采用体外、体内模型进行抗肿瘤活性筛查，对具有抗肿瘤活性的piRNA，从细胞生物学、分子生物学和表观遗传学三个方面深入探究其作用机理。本项目若能顺利实施，不仅可以发现新的活性物质用于肿瘤治疗，减轻患者痛苦、延长患者寿命，更可能从源头上对肿瘤细胞进行重编程，彻底根除其对人类生命的危害。

Exploring anti-tumor piRNA from bovine oocytes and the study on their action mechanism.It is a promising researching area to develop anti-tumor bioactive substances from animal resources. The applicant has found in previous research work that bovine oocyte extract can epigenetic reprogram H460 human lung cancer cells, and that the piRNAs traveling from extract into H460 cells play an important role in this process. These results suggest that the bovine oocyte extract piRNAs contain new anti-tumor active molecules, worthy of deep exploration. This project is aimmed to screen anti-tumor activity of the piRNAs traveling from extract into H460 cells, using in vitro and in vivo models. The mechanism of anti-tumor piRNA will be studied from cell biology, molecular biology and epigenetics. The project can not only find new rich resources for anti-tumor drug development, but also promote the fusion of animal science, oncology, and epigenetics, deeping the recognization of biology process.