氧化胁迫是不同逆境胁迫导致作物大规模减产的共同次生胁迫之一，改善作物氧化胁迫耐受性有望提高作物的广泛适应性。然而，迄今有关水稻品种间氧化胁迫耐受性差异关键调控基因的鉴定还没有相关报道。在前期研究中，我们利用高世代回交群体克隆到调控水稻品种间氧化胁迫耐受性差异的关键基因RT1。基于遗传互补株系、功能丧失突变体、过表达转基因株系、以及近等基因系的综合分析，我们证实RT1能有效的改善水稻的氧化胁迫耐受性及多个逆境胁迫的耐受性。本项目在此基础上，拟进一步深入分析RT1调控水稻氧化胁迫耐受性的分子机制，包括阐明RT1对上游氧化胁迫信号的响应机制，解析RT1互作蛋白RIP1的生物学功能，明确RT1下游抗氧化胁迫的转录调控网络，分析RT1对不同胁迫信号途径的调控，及评估RT1在水稻生产上的应用潜力。

Oxidative stress is one of the common secondary stresses of various environmental stresses which can dramatically reduce crop yield. It is expected to increase the wide adaptability of crops by improving their oxidative stress tolerance. However, the molecular mechanisms underlying difference in oxidative stress tolerance among rice varieties remain elusive. In our previous study, using the advanced backcross segregation population, we successfully cloned a critical gene RT1 which determines the difference in oxidative stress tolerance among rice varieties. Based on the comprehensive analysis of genetic complementation lines, loss-of-function mutant, overexpression transgenic lines, and near isogenic line, we confirmed that RT1 can effectively improves the oxidative stress tolerance and tolerance to several other stresses in rice. On these basis, we intend to further investigate the molecular mechanisms underlying RT1-mediated antioxidant response in this project, including: to elucidate the mechanism in which RT1 responses to upstream oxidative stress signaling, to dissect the biological function of RT1-interacting protein RIP1, to illustrate the anti-oxidative transcriptional regulation network downstream of RT1, to analysis the regulation of RT1 on different stress signal pathways, and to assess the potential application of RT1 in rice production.