紫外光UVC灭活输水系统沼蛤幼虫的机理研究

天然水环境中的沼蛤随水流入侵到输水系统，输水系统的构筑物和设备出现严重的沼蛤生物污损，导致阀门难以启闭并堵塞管道。沼蛤生物污损影响输水系统供水安全，迫切需要解决。申请人的研究基础证明，紫外光(UVC)灭活沼蛤幼虫是有效并可行的减少水中沼蛤生物总量的方法，该方法能显著地降低沼蛤生物污损的附着密度。为了阐明UVC灭活沼蛤幼虫的机理并提高效能，本课题采用荧光实时定量PCR、代谢组学以及DNA损伤拉曼光谱分析等技术，研究沼蛤幼虫在不同UVC辐射强度下的响应机制和沼蛤幼虫生理生化指标的变化规律，通过结合沼蛤幼虫遗传物质损伤的修复时间及其体内的紫外光敏感基因表达水平的变化，从分子生物学的角度揭示UVC灭活沼蛤幼虫的机制，并探究UVC辐射灭活沼蛤幼虫有效灭活时长与效能。通过系列研究，为UVC灭活沼蛤幼虫从而防止沼蛤生物污损奠定理论研究基础和相关科学方法。

Since the imigration of Limnoperna fortunei into long distance water system (LDWS), the biofouling, which formed by L.fortunei, amassed a huge amount on the structure and machinery in LDWS. L.fortunei’s biofouling make valve ineffective, clog pipelines, and hinder the operation of LDWS.Our research show that ultraviolet C is one of the most effective and feasible method to kill the veliger of L.fortunei. Due to the reduction of L.fortunei’ biomass, the density of L.fortunei’s biofouling was appeared to be decrease significantly. To upgrade the inactivation effect on L.fortunei’s veliger, this study is set up to illustrate the response of L.fortunei’s veliger on different radiation of ultraviolet c, consolidating with the change rule of physiological feature and biochemical parameter via metabolic fingerprinting analytical technique. According to genetic material damage repairing time and the level of ultraviolet light sensitive genes expression, the radiate time and intermittent time of ultraviolet light on the lethal of L.fortunei’s veliger is explored. Thus, this study can reveal the response mechanism of L.fortunei’ veliger to ultraviolet c from molecular biology, provide an effective method and lay theoretical foundation on the control of L.fortunei’s biofouling via ultraviolet c.