心血管材料植入引发的表面凝血反应及内皮功能紊乱常常导致植入的失败。通过表面改性赋予材料多功能生物活性具有十分重要的科学意义。本项目从仿生内皮功能角度出发，模拟贻贝足丝蛋白组成，在材料表面制备具有催化一氧化氮（NO）原位再生释放功能的载铜涂层，通过涂层中多酚官能团含量、铜的装载量及释放行为的控制与研究，探究涂层在原位催化血液内源性NO供体分解释放NO的能力，并进一步在材料表面固定CD34抗体分子，开展心血管材料内皮化修复的研究。研究涂层在催化NO释放及促进原位内皮化等多功能协同作用实现的影响因素。通过项目的研究开展，将在心血管材料表面生物功能及生物应答调控方面取得进一步认识，并建立起基于仿生贻贝的载铜涂层制备模型，为心血管材料表面生物多功能化的研究与应用提供研究基础

Surface coagulation and endothelial cell dysfunction after implantation frequently caused the failure of vascular implants. Surface modification techniques have important scientific significance and could provide multi-functional bioactivities on material surface. Inspired by endothelial cell function, this project is focusing on preparing copper-incorporated mussel-inspired coatings with the function of in-situ catalytic generation of nitric oxide (NO). The surface phenol group content, copper-incorporated amount and release behavior were further studied to investigate the ability of coatings on decomposing endogenous NO donor to release NO. Anti-CD34 antibody was further immobilized on the surface for the study of enhanced in-situ re-endothelialization. The mechanisms of the synergetic effect of NO generation and in-situ endothelialization will be investigated. By this project, new knowledge on regulating and controlling of surface biological function and response will be achieved. A model of preparing mussel-inspired copper-incorporated polyphenol coating will be established and could provide foundation to the research and applications of bio-multifunctions on cardiovascular material surface.