热带种子高温耐性的生理学基础与生态学意义

毁林开荒和雨林片断化引起林内温度升高，物种更替，导致一些热带雨林原生植物种类消失，外来植物入侵。项目以种子的高温耐性为切入点，分析和检测不同植物类群种子的高温耐性，研究种子高温耐性的生态学意义；通过高温胁迫处理前后种子或胚（胚轴）的活力变化与细胞膜完整性变化、超显微结构变化、抗氧化酶系统变化之间的相关性研究，探索种子高温胁迫伤害的机理；通过种子高温耐性和种子热稳定蛋白及不饱和脂肪酸的含量与成分之间的相关性分析，研究种子高温耐性的生物化学与分子生物学基础。研究高温胁迫对热带种子萌发的影响及其机理，寻找与种子高温耐性有关的生化指标和生物大分子，鉴别热带雨林中对高温胁迫敏感的植物类群，评估外来植物潜在的入侵风险，丰富种子生物学理论，为热带雨林和生物多样性保护提供指导。发表研究论文5-8篇，其中3-4篇被SCI期刊收录。培养研究生2-3名。

Deforestation and rainforest fragmentation lead to temperature increase inside forest, and subsequent species shift and disappearance of some climax species, with invasion of alien species. Taking seed high-temperature tolerance as a connecting point, this project will reveal ecological significance of high-temperature tolerance in tropical seeds through comparison of high-temperature tolerance among different plant groups, study the relationship between changes in seed viability and alternation of embryonic cell membrane integrity, cellular ultrastructure, efficiency of antioxidant system to investigate mechanisms of high-temperature injury in seeds, analyze the linkage between seed high-temperature tolerance and their heat-stable proteins and fatty acids to study biochemical and molecular biological base of seed high-temperature tolerance. To investigate the effects of temperature increase on seed germination in tropical rainforests and its mechanisms, seek the biochemical index and specific proteins corresponding with seed high-temperature tolerance, identify the taxa sensitive to high temperatures, evaluate the invasive risk of alien plants, enrich the theory of seed biology and provide reference to tropical rainforest and biodiversity conservation. Five to eight published papers are expected, of which three to four on SCI journal. Two or three graduate students involved.