在水稻育种中，株型是决定产量的重要因素，卷叶作为决定株型的重要农艺性状之一越来越受到育种家们的重视。但是，水稻叶片卷曲的分子机制仍不明确。前人研究表明HD-Zip IV类转录因子Roc5对于叶片卷曲起着重要的作用。本研究前期通过聚类分析和序列比对，鉴别得到Roc5的同源蛋白Roc8。Y2H和BiFC实验表明Roc8能够与Roc5互作，验证了Roc转录因子间能形成异源二聚体。遗传转化实验发现，该基因过表达和敲除分别导致叶片向近轴面和远轴面卷曲，证实其对于叶片的卷曲及形态建成同样起着重要的作用。本研究拟利用分子生物学和遗传学等实验方法，从基本特性分析，水稻中的功能验证以及与Roc5共同调控水稻叶片卷曲等方面对Roc8展开更为深入的研究，以期揭示Roc8调控叶片卷曲的分子机制。本研究将有利于进一步阐明该类转录因子对于水稻叶卷控制及叶片形态建成的分子机制，为改良水稻株型提供新的基因资源和理论依据。

In rice breeding, leaf rolling is receiving considerable attention as an important agronomic trait of plant architecture. However, little has been known on the molecular mechanism of rice leaf rolling. Previous study had reported that the Homeodomain Leucine Zipper Class IV transcription factor Roc5 controlled leaf rolling in rice. We isolated Roc8, a homologous protein of Roc5, by phylogenetic analysis and sequence analysis. It has been proved that Roc8 can interact with Roc5 by Y2H and BiFC analysis, which confirms that Roc proteins can form heterodimers in rice. Transformation analysis has shown that overexpression of Roc8 induces adaxially curled leaf, while knock-out of Roc8 causes abaxially rolled leaf, indicating that Roc8 plays an important role in rice leaf rolling and morphogenesis as well. By genetics and molecular biology methods, we plan to study the gene features and the function in rice, and further explore the leaf rolling regulation by the interaction between Roc8 and Roc5 to investigate the function and molecular mechanisms of Roc8 in leaf rolling regulation. This study may be beneficial for expanding our knowledge on the biological function of the Roc family proteins in rice leaf rolling and morphogenesis, and providing new genetic resources and theoretical basis for improving the plant architecture in rice.