巨噬细胞极化在痛风中的作用及干预策略研究

目前认为原位巨噬细胞启动痛风性关节炎的急性炎症，而单核/巨噬细胞如何参与急性痛风炎症的缓解仍需进一步研究。本课题将在前期MSU刺激大鼠踝关节以及小鼠足掌、腹腔模型初探的基础上，结合文献报道，提出原位巨噬细胞不仅是启动急性痛风性关节炎的主要参与者，对炎症的缓解也有重要作用，并进一步探索外周血募集的单核/巨噬细胞在急性痛风性关节炎中的作用。同时本课题将对Sirt1发挥预防急性痛风性关节炎作用的靶细胞及机制做进一步阐述，提出Sirt1/LKB1/AMPK通路可能通过影响巨噬细胞极化发挥抑炎作用，为预防、治疗急性痛风性关节炎提供新的治疗靶点。

Resident macrophages are thought to initiate acute inflammation of gout, however, how macrophages participate in the resolution remains unclear. Based on the primary investigations of MSU induced inflammation of rat ankles, mouse peritoneal cavities and mouse paws, our study will focus on the impact of resident macrophages in the resolution phage and the role of recruit monocytes/macrophages in acute arthritis of gout. Our study will investigate target cells and mechanisms of Sirt1 in prevention of acute arthritis of gout and explore the pathway Sirt1/LKB1/AMPK in polarization of macrophages to provide new targets for prevention and treatment of acute gout attack.