叶绿体外膜转运蛋白(Translocons at the outer envelope membranes of the chloroplast,TOC) 是一种GTP酶，负责调控细胞核编码的蛋白质识别与最初转运。叶绿体外膜转运蛋白-叶绿体内膜转运蛋白（TOC-TIC）导入途径的发现，引起研究者对叶绿体转运蛋白的功能及转运机制的研究兴趣。本项目基于目前拟南芥、豌豆TOC GTP酶的分子水平研究基础，以番茄(Solanum lycopersicum)为研究材料，明确6个番茄TOC GTP酶基因（slToc159-1，slToc159-2，slToc159-3，slToc159-4，slToc34-1和slToc34-2）的功能，分析TOC GTP酶转运蛋白的功能及转运特性，以获得更为精确的蛋白转运模式，也为高光和番茄育种工作提供潜在价值。

The translocon at the outer envelope membrane of chloroplasts (TOC) complex is responsible for recognizing preprotein and initiating import. Since the discovery of the TOC-TIC import pathway, there has been a great deal of interest in determining the functions of the components of the import machinery. Based on molecular study of TOC GTPase in Arabidopsis and pea, In this project, we identify the function of six the Toc GTPases from tomato, analyze transport specialization of a representative photosynthetic protein (the small subunit of Rubisco) and a representative non-photosynthetic protein (p E1a) into chloroplasts isolated from tomato leaves and chromoplasts isolated from red tomato fruit, obtain a more accurate and scientific protein transport model, in order to provide a new breeding direction and potential value for the future high photosynthesis breeding of Tomato.