miR-570/B7-H1/PD-1通路介导的免疫抑制性环境在肝癌休眠中的作用及健脾化瘀方的干预研究

肝癌术后休眠是近来的研究热点，但其机制不明。基于前期基础及其他学者的研究，我们认为miR-570调控的B7-H1/PD-1通路通在肝癌细胞术后休眠的中有重要作用。本项目拟以肝癌术后休眠/复发小鼠模型为研究对象，运用小动物PET/CT成像技术及qRT-PCR、Western blot、miRNA mimics等技术，先研究健脾化瘀方对肝癌术后休眠、术后复发模型小鼠体内miR-570、B7-H1、B7-H1 mRNA、AFP mRNA的表达水平及脾脏、血液中的CD4+、CD8+T淋巴细胞亚群表达情况及其比值；再从体外研究健脾化瘀方对肝癌术后休眠、术后复发模型小鼠脾淋巴细胞对正常表达miR-570和过表达miR-570的肝癌细胞杀伤活性的影响。通过上述研究阐明miR-570/B7-H1/PD-1通路介导的免疫抑制性环境在肝癌休眠中的作用及健脾化瘀方的作用。

Postoperative recurrent of liver cancer was a recently research hotspot, but the mechanism of the dormancy was not clear. Based on other scholars' and our preliminary research, we suspect the miR-570/B7-H1/PD-1 pathway plays an important role in the dormancy of liver cancer. We choose the small animal PET/CT imaging technology, qRT-PCR, Western blot and miRNA Mimics technologies to research tumor recurrent on the mice models. Firstly, we research the influence of the Jianpihuayu decoction on the miR-570, B7-H1, B7-H1 mRNA expression levels and the CD4+, CD8+ T lymphocyte subsets expression and their ratio on the tumor dormancy/recurrent models. Then, we research the influence of the Jianpihuayu decoction on the tumor killing activity of the spleen lymphocytes of the tumor dormancy/recurrent models to normal and high expressive miR-570 H22 cell. This study will reveal the role of miR-570/B7-H1/PD-1 pathway mediated immunosuppressive environment in the tumor dormancy/recurrent and the effect of Jianpihuayu decoction.