对虾白斑综合症病毒（WSSV）是我国对虾养殖业的主要病原之一，迄今还没有有效的防治方法，以病毒关键蛋白为靶点研发高效低毒的抗病毒药物是WSSV防治的一个重要途径。WSSV编码dUTPase，该酶水解dUTP为dUMP和焦磷酸，从而防止dUTP错误掺入DNA中，对保证病毒基因组的稳定性有重要作用，是理想的抗病毒药物靶点。然而，它的三维结构至今尚未得到解析，阻碍了以其为靶点的抗病毒药物设计。本项目拟解析WSSV和对虾dUTPase的晶体结构，为以WSSV dUTPase为靶点的药物设计提供全面准确的结构信息；还将通过基于结构的药物设计方法设计WSSV dUTPase的特异性抑制剂，评价其抗病毒活性，为研发高效低毒的抗WSSV病毒药物提供前期基础。本项目的研究内容对防治WSSV和保障我国对虾养殖业的健康持续发展有重要意义，而且，本项目引入基于结构的药物设计方法，为现代渔药的研发提供了新手段。

White Spot Syndrome Virus (WSSV) is one of the main pathogens of shrimp farming industry, with no effective control methods available so far. The development of potent and low toxicity antiviral drugs targeting key viral proteins is an important measure for WSSV prevention and treatment. WSSV encodes a dUTPase, which hydrolyzes dUTP to dUMP and pyrophosphate. The enzyme prevents dUTP misincorporation into DNA, maintaining the viral genome stability, and thus serves an ideal antiviral target. However, its three-dimensional structure has not yet been solved, which hinders the antiviral design targeting this enzyme. The project intends to determine WSSV and shrimp dUTPase crystal structures, providing complete and accurate structure information to facilitate drug design activity. We also intend to use structure-based drug design approach to design specific WSSV dUTPase inhibitors，and further evaluate their antiviral activity, providing a basis to develop potent and low toxicity anti-WSSV drugs. The project is important for treating WSSV, contributing to the healthy and sustainable development of China's shrimp farming industry. Furthermore, the project introduces structure-based drug design approach, providing a new means in modern fishery drug development.