microRNA-143/145调控Th9细胞抑制恶性腹腔积液的机制研究

Th9细胞促进恶性腹腔积液(MA)中发展，miR-143/145在MA中呈低表达并参与一些Th细胞的调控。迄今对miR-143/145与Th9细胞的关系及其在MA中作用机制仍知之甚少。我们预实验结果提示miR-143/145在Th9细胞调控和MA发展中起重要作用。但其作用机制还有待进一步探讨。为此，我们提出假说：miR-143/145可能通过调控Th9细胞影响MA发生发展。为了验证这一假说，我们将通过小鼠Th9细胞，肝癌细胞、MA模型，采用real-time PCR、Western-blot、脂质体转染、RNA干扰等手段，从分子、细胞、组织以及动物整体水平等多方面探讨miR-143/145在MA发生中的重要作用，明确miR-143/145通过调控Th9细胞影响MA发生发展的机制。本研究将从miR-143/145这个新视点为揭示调控Th9细胞对MA作用的机制，为MA防治提供新的思路。

Th9 cells promote the development of malignant ascites (MA); miR-143/145 present low expression in MA and they participate the regulation of some Th cells. Currently, little is known about the relationship of miR-143/145 and Th9 cells and their role in MA. Our preliminary experiment suggested that miR-143/145 is critical in the regulation of Th9 cells and may play important role in the development of MA. However, the exact mechanism of this role has yet to elucidate. Therefore, we hypothesis that miR-143/145 suppress the development of MA through the regulation of Th9 cells. In order to validate this hypothesis, we will use Th9 cells from mice, hepatocellular carcinoma cell lines, MA model, and the methods such as real-time PCR, Western-blot, Lipofectamine transfection, siRNA, to explore the role of miR-143/145 in MA, and verify the mechanism of miR-143/145 suppress the development of MA through regulation of Th9 cells from the levels of molecule, cells, tissue and animal model. Our study will give an insight into the mechanism of miR-143/145 suppress MA through the regulation of Th9 cells, and provide new idea for the treatment of MA.