Stomatin基因在肺癌中的生物学功能及机制研究

肺癌是恶性肿瘤中发病率和死亡率最高的，尽管治疗方法不断进步，但肺癌患者总体生存率依然很低。因而迫切需求发现新的更有效的肺癌生物标志物和治疗靶点。本项目前期研究发现Stomatin在肺癌组织中表达显著高于正常组织，且Stomatin影响肺癌细胞增殖凋亡、Glut1胞膜定位及糖代谢相关信号通路AKT的激活。基于这些研究结果，我们将对Stomatin影响肺癌细胞增殖、凋亡及糖代谢的机制做深入探究。前期探索Stomatin与肺癌进展相关性的实验中发现，Stomatin还能影响细胞运动迁移能力并在TGF-β诱导EMT过程中表达下调，但机制尚不清楚。本项目将继续扩大肺癌临床标本，以期确定Stomatin同肺癌转移相关性，并从细胞及动物水平揭示Stomatin调控细胞运动迁移能力的机制。依据文献查阅及前期实验结果，我们推测Stomatin在肺癌发生发展过程中发挥重要作用。

Lung cancer is the malignancy with the highest disease incidence and mortality rate. Despite advances in treatment, the overall survival is still low in lung cancer patients. Therefore, there is an urgent need to find new and more effective cancer biomarkers and therapeutic targets. In our previous study, we found that Stomatin was significantly higher in lung cancer, and its expression could influence cell proliferation, apoptosis, the membrane localization of Glut1 and glucose metabolism related signaling pathway AKT activation. Based on these findings, we will in-depth study how Stomatin affects lung cancer cell proliferation, apoptosis and glucose metabolism. Besides influencing the occurrence of lung cancer, we also found the association between Stomatin expression and cell migration, and Stomatin was downregulated during TGF-β induced epithelial-mesenchymal transition (EMT), but the underlying mechanism is unclear. We will expand the sample size, determine the correlation between Stomatin and lung cancer metastasis, and uncover how Stomatin controls the cell movement in vitro and in vivo. Based on literature review and previous results, we infer that Stomatin may play a critical role in the occurrence and development of lung cancer.