本项目申请者团队前期研究发现脂质转运障碍导致脂代谢失衡，脾虚生痰是脂代谢紊乱的基本病机，因而认为脂质转运是“脾主运化”在水谷精微转输布散的具体体现之一。Caveolae是细胞表面参与脂质转运的特殊结构，其介导的脂质转运与脂代谢关系密切。本项目拟在前期研究基础上，以脂代谢紊乱金黄地鼠模型为对象，并对其进行健脾化痰的二陈汤干预后，观察脂代谢变化情况：（1）采用Real-time PCR和Western Blot技术分别检测地鼠脂肪和肝组织中Caveolae介导的脂质转运因子mRNA和蛋白的表达水平；（2）对地鼠脂肪和肝组织的冷冻切片进行油红染色和免疫荧光染色，观察二种组织细胞中脂肪的含量和Caveolae介导的脂质转运因子的定位。目的在于阐明脾虚生痰与Caveolae介导的脂质转运的密切联系，揭示健脾化痰法调节脂代谢紊乱的分子生物学机制，为进一步探究脾主运化在脂质代谢中的物质基础提供思路。

The previous researches pointed out that lipid transport barriers lead to lipid metabolism imbalance, and the basic pathogenesis is spleen deficiency. Lipid transport is the embodiment of spleen governing transportation and transformation for lipid to nourishing the whole body. Recent studies have shown that caveolae are cell surface plasma membrane invaginations, and caveolae-centered lipid transport is closely related to lipid metabolism. Therefore, based on previous work, golden hamster will be fed by high fat diet to establish the experimental lipid metabolism disorder model. After traditional Chinese prescription interventions, Real-time PCR and Western Blot will be used to detect the expression of caveolae-mediated lipid transporters in adipose and liver tissue of these golden hamster. Meanwhile, we will observe the localization of caveolae-mediated lipid transporters in adipose and liver tissue cells using immunofluorescence technique. The purpose is to clarify the close ties between spleen deficiency and caveolae-mediated lipid transport, and investigate the molecular mechanism of invigorating spleen to remove phlem regulating lipid metabolism disorders, and further enrich the scientific meaning of "spleen governing transportation and transformation".