生物体的脂代谢稳态平衡是各个组织协调作用的结果，脂稳态的紊乱会导致代谢综合征如肥胖、糖尿病、脂肪肝的发生。Cidea和Fsp27是最近发现的在脂类代谢中起着重要作用的蛋白。之前的研究都关注在单个蛋白Cidea或者Fsp27在脂肪组织中的功能研究，但在褐色脂肪组织及脂肪性肝脏中同时有Cidea和Fsp27的表达，并且Cidea蛋白在Fsp27缺失的白色脂肪组织中也有表达。Cidea和Fsp27两个蛋白是如何共同作用调控肥胖小鼠脂肪组织和肝脏脂类代谢的尚不清楚。我们拟利用ob/ob/Fsp27-/-/Cidea-/-小鼠，研究在肥胖状态下脂肪组织和肝脏中Cidea和Fsp27对脂积累的影响，以及Cidea和Fsp27缺失后的脂肪组织和肝脏对机体整体代谢状态的影响。并在细胞和分子水平上研究Cidea和Fsp27双缺失对脂储存的影响。

Lipid homeostasis represents the relatively stable levels of lipids in circulation and in various tissues including liver, muscle and adipocytes . Lipid homeostasis is a result of the coordination of various tissues. Disrupt lipid homeostasis will lead to metablic diseases such as diabetes obesity and hepatic steatosis. Cidea and Fsp27, members of the Cide family proteins, are lipid-droplet-associated proteins that has emerged as important regulator of lipid storage in adipocyte. Both Cidea and Fsp27 knockout mice has lean phenotype and reduced adipose tissue. Previous research mainly focus on the function of either Cidea or Fsp27. However,. Cidea and Fsp27 are both highly expressed in brown adipose tissue and steatotic liver. Cidea is also upregulated in the white fat tissue of Fsp27 deficient mice. It is important to study the function of both Cidea and Fsp27 in lipid metabolism. In this proposal, we will investigate the mechanism of Cidea and Fsp27 in lipid metabolism using ob/ob、ob/ob/Fsp27-/-、ob/ob/Cidea-/- and ob/ob/Fsp27-/-/Cidea-/- mice. We will use techniques such as molecular and cellular biology to further elucidate the role of Cidea and Fsp27 in liver and adipocyte lipid metabolism.Furthermore, We will investigate the interaction between Cidea/Fsp27 and ATGL in regulating lipid storage. Overall, our study will provide sigificant insight in Cidea and Fsp27-controlled lipid homostasis and the mechaism of the development of metabolic disease.