癌症是威胁人类健康的主要疾病之一，而天然产物是抗癌药物的重要来源。赶山鞭（Hypericum attenuatum）为藤黄科金丝桃属重要的传统药用植物，目前为止几乎没有其化学成分系统研究的报道。我们前期对其次生代谢产物进行了初步研究，发现了27个多环多异戊烯基间苯三酚（PPAPs）类化合物，其中包括11个新的PPAPs，这些新化合物大多具有一定的细胞毒活性。最近，对这些新的PPAPs进行广泛的活性筛选发现，其中部分化合物具有较好的己糖激酶-2抑制活性。本项目拟对华中大别山地区的赶山鞭化学成分进行深入的研究，对富含PPAPs的小极性部分进行深入的、系统的分离纯化，以期发现更多结构新颖的PPAPs类化合物。通过对所得大量PPAPs化合物己糖激酶-2抑制活性的筛选和细胞毒活性筛选，寻求活性优良的PPAPs，进一步阐明其抗肿瘤活性作用机制，为后续结构修饰和最终开发抗癌药物提供先导化合物和科学依据。

Cancers figure among the leading causes of morbidity and mortality worldwide. Natural products continue to play a highly significant role in the drug discovery and development process. Hypericum attenuatum (Guttiferae) is an important traditional medicinal plant, and there is no systemically phytochemical investigation about it. Our previous phytochemically investigation of Hypericum attenuatum (Guttiferae) led to the isolation of 27 polycyclic polyprenylated acylphloroglucinols (PPAPs), including 11 new ones, and most of them exhibited cytotoxic activities. This study focuses on the chemical constituents of H. attenuatum of Dabie-Mountain areas, which will be systemically investigated in searching for structural intriguing PPAPs. Based on these isolated PPAPs, their inhibitory activities of HK-2 and their cytotoxic activities will be scanned, and then, their mechanism of anti-tumour will be investigated and illuminated, which will greatly benefit the followed medicinal development of PPAPs.