新疆是我国唯一的海岛棉产区，持续高温给棉花产量造成了极大损失。研究表明，花药绒毡层是对高温非常敏感的组织，其提前降解可能是直接造成花粉败育的主要原因。进一步分析发现，以往研究花粉败育多侧重于对雄性不育材料在花粉萌发后→受精过程完成一小段时期来揭示其原因的，而在持续高温胁迫下耐高温品种在花粉形成过程中前体物质花药绒毡层变化与花粉败育的关系还少见报道，特别是其不同时期激素水平、核DNA（RNA）含量等相关指标的动态消长与绒毡层及育性的关系还未见报道。本研究以耐高温品种新海35号和温敏品种苏11为研究材料，在模拟田间高温条件下，采用切片技术和流式细胞术，以减数分裂期为中心，通过追踪减数分裂期至开花期间花药绒毡层组织细胞的发生及消亡，明确不同阶段内源激素和核DNA（RNA）动态消长规律，建立各指标间与花粉败育的相关关系，以有助于了解高温条件下绒毡层异变对海岛棉蕾铃脱落的细胞学及生理机制问题。

Xinjiang is only island cotton producing area in China,and continuous high temperature for cotton production caused great losses.Studies had shown that Tapetum was very sensitive to high temperatures organization whose early degradation maybe a direct result of the main reason for pollen abortion. Further analysis revealed that pollen sterility multiple previous studies focused on sterile material from fertilization pollen germination to completed after a short period to reveal its causes,but was rarely reported that the relationship of pollen sterility and the precursor tapetum change in resistant high temperature varieties under the continuous high temperature stress in pollen, and especially in the dynamic relationship between growth and decline tapetum and fertility hormone levels in different periods of its nuclear DNA (RNA) content and other related indicators had not been reported.In this study,we will use Xinhai 35 of high temperature resistant varieties and Su- 11 temperature-sensitive species for the study of materials under high temperature conditions in the simulation field , using the slicing technique and flow cytometry to meiosis as the center, through meiosis to track during flowering tapetum cells occurred and the demise of the different stages of endogenous hormones clearly and nuclear DNA (RNA) dynamic ebb and flow rule, establishing relationship between the index and pollen sterility.In order to help to understand Tapetal cytology and physiological mechanisms of anther tapetum mutation Under high temperature conditions on island cotton abscission.