Kisspeptin54对乏情季节滩羊繁殖的作用及其机制研究

Kisspeptin是Kiss1基因的产物，对GnRH的释放具有明显的调控作用，与动物季节性繁殖关系密切。宁夏滩羊为季节性发情动物，产羔率较低。为了诱导滩羊在乏情季节发情、排卵，本研究通过给乏情季节雌性滩羊单次重复和连续静脉注射kisspeptin54 48h或72h， 然后kisspeptin54共培养垂体细胞、卵母细胞、卵巢颗粒细胞，通过RIA、ELISA检测激素水平、流式细胞技术检测细胞周期变化、q-PCR和Western blot检测相关基因及蛋白表达来明确kisspeptin54对乏情季节滩羊繁殖的作用，阐明其在下丘脑-垂体-性腺轴上调控乏情季节滩羊繁殖的机制，为开发滩羊乏情季节繁殖调控新技术提供理论依据和前期技术支持。

Kisspeptin is the product of Kiss1 gene,it is closely associated with season reproduction apart from regulating GnRH release in animals. Tan-sheep belongs to seasonal oestrus animal with low lambing rate in Ningxia province. In order to induce oestrus and ovulation of Tan-sheep in anestrous season, kisspeptin54 will be injected to female Tan-sheep by single repeated injection or continuous infusion lasting 48h or 72h, then pituitary cells, oocyte and ovarian granulosa cells are co-cultured with kisspeptin54,respectively.The role of kisspeptin54 on reproduction of Tan-sheep will be revealed and the mechanism of kisspeptin54 regulating reproduction in hypothalamic-pituitary-gonadal axis will be explored in anestrous season by detecting hormone levels，cell cycle changes, the expression of related genes and proteins with RIA, ELISA,flow cytometry, q-PCR and Western blot. It will offer theoretical basis and early technical supports for developing a novel reproductive technologies of Tan-sheep in anestrous season.