前期研究证实安肠汤对IBS有明显的临床疗效，其主要通过降低内脏高敏感性而发挥效应，而内脏高敏感性主要与疼痛信号转导的外周敏化和中枢敏化有关，因此探讨内脏疼痛信号转导通路中外周敏化和中枢敏化关键靶点十分重要。研究表明，PKCγ/ERK1/2/MAPK信号通路中PKCγ,ERK1/2,mGluR5, NR2BR靶点在IBS的内脏疼痛信号激活、转导、调控中据有关键位置，与外周敏化和中枢敏化关系密切。项目拟通过建造肝郁脾虚IBS-D的大鼠模型，以利用Western bloting，钙离子成像和电生理技术观察安肠汤治疗前后不同级别神经元组织的PKCγ,ERK1/2,mGluR5, NR2BR蛋白表达，细胞内钙离子浓度的改变，大肠离体平滑肌肌张力的变化，阐明内脏高敏感性和内脏疼痛信息转导通路敏化的关键环节，从分子水平上探讨中药安肠汤干预IBS的主要靶点。

In the early days reasearch has proved Anchang decoction has obvious curative effect in treating IBS, and this mechanism was to reduce visceral hypersensitivity, which mainly related to peripheral sensitization and central sensitization of pain signal transduction. So invesitigation the key target of peripheral sensitization and central sensitization of visceral pain signal transduction pathways become an important problem. Some research shows that PKCγ,ERK1/2,mGluR5, NR2BR targets play important roles in PKCγ/ERK1/2/MAPK signal pathway to activate, transduce and regulate visceral hypersensitivity. Based on these findings, the project group intended to build a Rat Model of Irritable Bowel Syndrome of liver depression and spleen deficiency. Using Western bloting technique, calcium imaging technique and electrophysiological technique to test the protein expression of PKCγ,ERK1/2,mGluR5, NR2BR, the change of intracellular calcium ion concentration and the muscular tension of large intestine smooth muscle in vitro in Different levels neuron tissues intervened by Anchang decoction between pre and post treatment, respectively. Through the above measures, we want to clarify the key link between visceral hypersensitivity and visceral pain signal transduction pathways sensitization, and illustrate the main target of IBS intervened by Anchang decoction in molecular level.