一种基于3D打印技术的组织工程亚微血管网络研究

构建与脏器相匹配的三维血管网络是代谢性组织工程脏器研究中亟需攻克的一个关键点。本研究拟以构建肝脏血管网络为目标。首先，将通过CT采集肝脏血管图像，结合数学建模和计算机图像处理技术，根据外形约束条件，建立肝脏亚微血管网络数学模型并进行血流动力学结构优化；其次，利用我们前期研究制备的光敏生物材料，采用3D打印技术制备相应的3D亚微血管网络构架；再次，通过细胞因子TSP1灌注、细胞种植、3D培养和体内植入实验，构建3D组织工程肝脏亚微血管网络；最后，通过高分辨率的同步辐射成像技术进行成像。本研究项目结合了数学建模，计算机图形处理，生物可降解光敏材料，3D打印技术和同步辐射成像技术，旨在将组织工程肝脏血管网络的精度从亚毫米级别提升为亚微米级别。如获成功，将为组织工程脏器的构建提供一种外形高适应，拓扑结构可控，血流动力学优化的3D亚微血管网络构建方法，促进代谢性组织工程脏器的研究。

Tissue engineering metabolic organs, as the hot topics of tissue engineering and regenerative medicine, provide solutions to organ shortage. At present, one of the most important challenges is a 3D microvascular network construction, which should be matching the tissue engineering organ structure. This research project focuses on human hepatic microvascular networks. Firstly, we plan to set up a bionic and hemodynamic optimized hepatic microvascular network model by the mathematical modeling and image processing technology. And then, we are going to build 3D metabolic organ microvascular network scalffold based on 3D printing technology. Finally, we plan to construct 3D tissue engineering microvascular network in vitro and in vivo, and observe the structure through the non-destructive and high resolution imaging technology of synchrotron radiation. This research project, combining the mathematical modeling, 3D printing, tissue engineering and synchrotron radiation imaging, aims to upgrade the tissue engineering hepatic vascular network from millimeter level to micrometer level. It will provide a solution to build microvascular networks of tissue engineering metabolic organs, and promote the research of tissue engineering organ forward.