生物胺的准确测定是海水鱼安全性评价的一项重要指标。目前，常用的基于色谱的方法能够客观、准确地反应多种生物胺的含量，但对样品前处理的要求高，操作繁琐、耗时费力。本项目围绕海水鱼中多种生物胺的快速同步检测，基于光电离离子迁移谱，采用时间分辨热解析进样，通过筛选试剂分子，利用试剂分子与生物胺之间的离子-分子反应增强生物胺的光电离响应，实现光电离离子迁移谱对生物胺的高灵敏度检测；采用离子高动能化技术，缩短反应区离子间的反应时间，消除多种生物胺在光电离离子迁移谱中的竞争性电离反应，构建基于光电离离子迁移谱的多种生物胺同步快速检测方法，并对几种典型海水鱼贮藏过程中生物胺的变化规律进行研究；利用TOFMS技术，对试剂分子形成的反应离子以及反应离子和样品分子之间的微观离子-分子反应进行分析，解析光电离离子迁移谱对生物胺的同步响应机制，为多种生物胺的同步快速检测奠定理论基础，为海水鱼安全性的快速评价提供新的技术手段。

Accurate determination of biogenic amines is an important index to evaluate the safety of marine fishes. Generally used methods based on chromatography could provide high accuracy, but suffer from limitations such as tedious preparation procedures, complexity and long analysis times. This project will employ photoionization ion mobility spectrometry for developing a rapid synchronous detecting method for a variety of biogenic amines in marine fishes combined with time-resolved thermal desorb sampling technology. Dopant would be used to enhance the sensitivity of biogenic amines through the ion-molecular reaction between reagent ions and biogenic amine molecular. High kinetic energy technology will be used to eliminate the competing ionization between biogenic amines by shorting the reaction time of ions in the reaction region, which is benefit for realizing the rapid synchronous detection of biogenic amines in photoionization ion mobility spectrometry. The developed method will be used to measure the changes of biogenic amines in several typical marine fishes during storage. The detection mechanism of biogenic amines by photoionization ion mobility spectrometry will be investigated by IMS-TOFMS technology by analyzing the micro ion-molecular reaction between reagent ions and biogenic amine molecular, which lays a theoretical foundation for the rapid and simultaneous detection of various biogenic amines.