ILP-2 是新发现的凋亡抑制蛋白，它对乳腺癌生长的影响尚未见报道。我们前期实验显示ILP-2 在乳腺癌（术前）患者血清及乳腺癌细胞中含量较高，据此推测ILP-2 可能与乳腺癌生长关系密切。本项目拟采用临床切除的乳腺癌及癌旁组织分析ILP-2 与乳腺癌生长的相关性；RNAi 下调乳腺癌细胞ILP-2 表达，RT-PCR、Western blot、MTT、流式细胞仪分析细胞生长变化；RT Profiler PCR Arrays研究ILP-2对Caspase-9、 Smac基因的作用；MCF-7等细胞共转染ILP-2 和Caspase-9、Smac 表达载体， Western blot、免疫共沉淀、BRET 研究ILP-2 与Caspase-9、Smac 的作用；建立人乳腺癌裸鼠皮下移植瘤模型，探讨ILP-2 作为乳腺癌基因治疗分子靶的可行性。本研究旨在为乳腺癌基因治疗寻找新靶点提供实验依据。

ILP-2( Inhibitor of Apoptosis Protein-Like Protein-2 ) is the newly discovered inhibitor of apoptosis protein. Our preliminary data evidenced a higher ILP-2 expression in the breast cancer (preoperative) patients′ sera samples and the breast cancer cells implying a relationship between this protein and breast cancer. So far, the exact role of ILP-2 in the breast cancer is still unknown. Based on our preliminiary data, we hypothesize that ILP-2 may play a pathogenic role in the breast cancer growth. The aim of the project proposed here is to analyze the role and molecular mechanism of the ILP-2 to the breast cancer growth.We are going to analyze the correlation of the ILP-2 expression and the breast cancer growth in the breast cancer tissue samples and the breast carcinoma sides tissues samples using real time RT-PCR, Western blot and immunohistochemistry technology. At the same time, in order to verify the authentic role of ILP-2 in the breast cancer, we will examine the growth of the human breast cancer cells in case of silencing of ILP-2 by RNAi technology.Also,the Human Breast Cancer RT2 Profiler? PCR Array will be applied to analyze the correlation of the ILP-2 gene and the Caspase-9 or Smac gene. Furthermore,immunoprecipitation will be applied to elucidate the protein-protein interaction between ILP-2 and Caspase-9,Smac,etc. by co- transfection of ILP-2 with Caspase-9 or Smac expression vector into the MCF-7 cells,etc.The interaction of ILP-2 and Caspase-9 or Smac will be also examined in the human breast cancer cells in a real time manner using bioluminescence resonance energy transfer technology (BRET). Finally, the human breast cancer xenograft in nude mice will be used to investigate the therapeutic potentiality of ILP-2 as a molecular target in the breast cancer treatment.