基于IL-33/ST2信号通路的瑞香素调控特应性皮炎小鼠肥大细胞活化的机制研究

特应性皮炎是一种免疫性皮肤病，近年来其发病趋势逐年增高。研究表明，该病类属于I型变态反应，其发病机制与肥大细胞活化密切相关。本课题在瑞香素调控免疫及抑制肥大细胞脱颗粒的相关文献报道的基础上，结合课题组前期研究结果，提出了“瑞香素通过调控IL-33/ST2信号通路发挥抑制特应性皮炎小鼠肥大细胞活化的药效作用”的假说。本项目采用Western blot、PCR、流式细胞术以及Transwell共培养等技术，在整体动物、细胞和分子三个层面上，考察瑞香素对特应性皮炎模型小鼠皮损组织的病理改变及肥大细胞数目变化、皮损处ILC2数目变化、外周血相关免疫因子及IgE含量的影响，对IL-33刺激骨髓来源肥大细胞脱颗粒活化及IL-33/ST2信号通路关键蛋白表达的影响；从IL-33/ST2信号通路入手揭示瑞香素治疗特应性皮炎的分子机制，为中药瑞香素的后续研究提供理论依据。

Atopic dermatitis is an immune skin disease, with the incidence trend increased in recent years. Studies have shown that this disease belongs to type I allergy, and the pathogenesis is closely related to mast cell degranulation. In this study, our group proposed the hypothesis" Through controlling the expression of main protein of IL-33/ST2 signal pathway, daphnetin can inhibit mast cell degranulation and play a therapeutic effect in atopic dermatitis”, based on the reports of daphnetin regulating immunity and inhibiting mast cell activation, combined with the results of our research group. In this study, we will use Western blot, PCR, flow cytometry and Transwell co-culture technology, and observe the indexes from the whole animal, cell and molecular levels. we investigated the changes of the lesion, the number of mast cells and ILC2 in the lesions, the level of immune factors and IgE in blood. The effect of IL-33 on bone marrow-derived mast cell activation and the expression of related proteins of IL-33/ST2 signaling pathway also will be tested. This project is to reveal the molecular mechanism of daphnetin in the treatment of atopic dermatitis from the IL-33/ST2 signaling pathway, with the purpose of providing new ideas and provide the theoretical basis for the follow-up study of daphnetin.