基于群体基因组学的孟氏隐唇瓢虫引进地适应性研究

孟氏隐唇瓢虫Cryptolaemus montrouzieri Mulsant原产澳大利亚，作为粉蚧类害虫的捕食性天敌，自1891年起被广泛引进到包括中国在内的全球多个国家和地区。但是，至目前还没有对引进孟氏隐唇瓢虫的生态风险作评估，对孟氏隐唇瓢虫在引进地的适应机制仍不清楚。本研究将基于新一代测序技术，从基因组的水平揭示孟氏隐唇瓢虫在人为引进后适应性的遗传机制。本研究将收集孟氏隐唇瓢虫的澳大利亚原产地种群、全球实验室和商品化饲养种群以及引进地定殖种群，采用RAD标记测序技术，从基因组里挖掘SNP位点，分析种群的遗传多样性和种群分化、种群的动态历史，以及分析表达基因的突变来推测选择压力水平及相关的生态因子，从而探讨该引进天敌的遗传变异模式与环境变化的关系，为引进天敌的合理安全使用提供理论依据。

Cryptolaemus montrouzieri Mulsant is native to Australia, and has been worldwide introduced as a biological control agent due to its ability to control mealybugs. However, its environmental risk as an introduced agent has not been evaluated, and its genetic pattern of local adaptation is still unclear. This research is aim to explore the genetic adaptation of the introduced Cryptolaemus montrouzieri in genome-level based on next-generation sequencing. The native, lab/factory reared and locally established populations will be collected and studied. The RAD sequencing will be used to discover SNP markers. The genetic diversity, divergence and demographic history will be analyzed. The genes under selection and related ecological factors will be identified. This research will provide theoretical basis for securely used of the introduced biological control agents.