马铃薯Y病毒（Potato virus Y，PVY）是危害马铃薯安全生产的重要病毒。种植抗病品种是防治马铃薯病毒病最根本有效的方法，但现有品种大都不抗PVY，因此培育抗PVY新品种是马铃薯生产中急需解决的问题。申请者在PVY HCpro复合体中筛选到RanGAP蛋白，超表达该蛋白能降低PVY在寄主中的积累水平，沉默该蛋白则提高PVY致病力，但其作用机制还不清楚。本项目拟鉴定与马铃薯RanGAP直接互作的病毒蛋白，明确RanGAP与PVY蛋白的互作网络；鉴定与马铃薯RanGAP互作的马铃薯蛋白，阐明RanGAP参与PVY侵染的信号通路；分析马铃薯RanGAP超表达或沉默对其它病毒侵染的影响、其它寄主RanGAP超表达或沉默对PVY侵染的影响。预期结果将阐明马铃薯RanGAP在PVY侵染过程中的作用机制，发现新的抗病毒靶标，为培育抗PVY马铃薯品种提供依据，也可为相关病毒的防治提供理论指导。

Potato virus Y (PVY) is one of the most destructive virus affecting potato production. Planting resistant cultivars is the most economic and efficient way to control potato viral diseases, however, most commercial potato cultivars are highly susceptible to PVY. Breeding new resistant potato cultivars becomes one of most urgent problems confronting potato production. Recently, the applicant has obtained a new HCpro interacting protein RanGAP using Co-IP. Over-expressing RanGAP reduced the accumulation level of PVY, while silencing Ran GAP enhanced the virulence of PVY. However, its mechanism during PVY infection remains unclear. In this proposal, we will screen and identify the RanGAP-interacting PVY and host proteins, study the signaling pathway regulated by RanGAP during PVY infection, characterize the effect of over-expressing or silencing RanGAP on the infection of other viruses infecting potato and dissect the role of RanGAP from other host during PVY infection. The expected results will elucidate the mechanisms of potato RanGAP protein involved in PVY infection, reveal new antiviral targets for the control of PVY and other related viruses.