腺苷信号介导电针预处理脑缺血耐受的机制

针刺作为脑中风康复常用的治疗方法，其作用机制至今仍不明确。课题组前期研究发现电针的脑缺血耐受效应可被腺苷A1受体拮抗剂逆转，同时电针正常大鼠百会穴除可上调脑的腺苷浓度及A1受体mRNA表达量，也上调穴位周围的腺苷浓度，由此提示腺苷信号可能参与了电针脑缺血耐受。据此课题组提出以下科学假说：腺苷信号介导电针的脑缺血耐受作用。本课题将采用siRNA技术使大鼠穴位周围和脑内的腺苷A1受体表达沉默，同时采用腺苷A1受体基因敲除小鼠建立MACO模型，运用TTC 染色、微透析法、Western-Blot、免疫组化、TUNEL、Real-time PCR，细胞转染等实验方法，分别从整体、组织、细胞、分子、基因等不同层面论证腺苷信号是否介导了电针的脑缺血耐受？阐明其机制是外周或中枢，还是两者共同参与？本研究成果将为电针治疗脑缺血性损伤的理论基础提供科学依据。

Acupuncture as a traditional Chinese technique in treatment for stroke, however the potential mechanism remain unknown. Our group has demonstrated that the cerebral ischemic tolerance induced by electroacupuncture was inhibited by adenosine A1 receptor antagonist. Furthermore, electroacupuncture contribute to upregulation of adenosine A1 receptor mRNA and adenosine concentration in native rats, indicating that adenosine signal mediate electroacupuncture preconditioning induced cerebral ischemic tolerance. SiRNA technique and adenosine A1 receptor gene knockout animal were used in our study, and to establish MACO model，the experimental methods including TTC staining, microdialysis, Western-Blot, Immunohistochemistry, TUNEL, Real-time PCR, cell transfection . Demonstration that whether adenosine signal mediate electroacupuncture preconditioning induced cerebral ischemic tolerance by various levels such as tissue,cell ,molecule and gene? To clarify its mechanism is peripheral or central, or both? This research provide a scientific basis for the electro-acupuncture treatment of cerebral ischemic injury.