细胞内吞金纳米棒和Gd@C82(OH)22纳米颗粒的分子机制研究

纳米技术正被广泛应用于生物医药、工业等领域，对纳米材料生物医学效应、安全性的研究显得日益重要。很多因素可以影响和调控纳米物质与细胞膜的相互作用及其在体内的分布，这为我们系统研究纳米材料的生物医学效应，并从中总结出其物理化学性质带来诸多挑战。本项目拟对细胞内吞表面修饰的金纳米棒和Gd@C82(OH)22纳米颗粒这两个问题进行理论研究，与具体实验工作相结合，集中讨论这两种纳米颗粒形貌、尺寸和表面理化性质对细胞内吞的影响。这两方面的研究将帮助我们更好地理解表面修饰的金纳米棒的细胞毒性和Gd@C82(OH)22低毒高效的肿瘤抑制效应中的内吞问题。另外，通过对细胞内吞这两种纳米颗粒的尺寸效应的研究，探索纳米材料生物医学效应理论研究的一个新领域：系统地探讨这两种纳米颗粒对细胞膜结构的影响，并与相关的实验结果进行比较，深入研究在各种因素作用下纳米颗粒尺度对不同内吞机制的选择和受限情况并得出最佳内吞尺度。

As nanomaterials are widely used in biomedical, industrial and other fields, the research on biomedical effects and nanosafety has become increasingly important. Many factors can influence and mediate the interaction of nanomaterial and cell membrane as well as its distribution in the living systems. Challenges exist as we study the biomedical effects of nanomaterials, and summarize its physical and chemical properties. This project aims to the theoretical study of the endocytosis of modified gold nanorods and Gd@C82(OH)22 nanoparticles, and combined with the specific experimental work, we focus on the effects of the morphology, size and surface physicochemical properties of the two types of nanoparticles on endocytosis. The study of the two aspects will help us better understand the endocytic issues of the cytotoxicity of modified gold nanorods and the effect of low-toxic high-efficient tumor suppressor Gd@C82(OH)22. In addition, we focus on size effect on the endocytosis of the two kinds of nanoparticles, which is a new field of the theoretical research on the biomedical effects of nanomaterials. We systematically explore their effects on the cell membrane structure and combine with the related experimental results. Furthermore, we study nanoparticle's size selection of different endocytic mechanisms and restriction under the influence of various factors, obtain the best size of endocytosis.