胆绿素通过LncRNA-H19调控miR-181b-5p影响Ems1治疗脑缺血-再灌注损伤的机制研究

炎症反应在脑缺血再灌注损伤（CIR）中发挥重要作用，在本项目组前期研究中发现：①胆绿素（BV）对CIR大鼠治疗作用与减少炎症反应有关；②同时利用全转录组测序，发现治疗后变化最明显的miRNA是miR-181b-5p，同时进行生物信息学预测其靶基因与List A;③取List A与mRNA测序结果取交集，发现靶基因中变化最明显的Esm1；④转录组测序中意外发现lncRNA-H19。经生物信息学预测其可以作为ceRNA,吸附内源性的miRNA-181b-5p，而影响其靶基因的表达。由此建立假说：胆绿素通过lncRNA-H19调控miRNA-181b-5p影响靶基因Ems1在CIR中发挥作用。我们将利用荧光素酶报告基因实验验证与Ems1的关系，同时利用RNA干扰技术从体内、体外论证其功能，从lncRNA-H19作为ceRNA的调控网络揭示胆绿素在治疗CIR中的新机制，为CIR的治疗提供新的思路。

The inflammatory response plays an important role in cerebral ischemia-reperfusion injury (CIR). In the previous study of this project group, it was found that:① Biliverdin (BV) is related to the reduction of inflammatory response in CIR rats;② After sequencing, it was found that the most obvious miRNA after treatment was miR-181b-5p, and bioinformatics predicted its target gene and List A; ③Take the intersection of List A and mRNA sequencing results, and find the most obvious Esm1 in the target gene.④Unexpected discovery of lncRNA-H19 in transcriptome sequencing. Bioinformatics predicts that it can act as a ceRNA, adsorbing the endogenous miRNA-181b-5p, and affecting the expression of its target gene. Thus, the hypothesis is established: biliverdin regulates miRNA-181b-5p through lncRNA-H19 and affects the target gene Ems1 to play a role in CIR. We will use the luciferase reporter gene assay to verify the relationship with Ems1, and use RNA interference technology to demonstrate its function in vivo and in vitro. From lncRNA-H19 as a regulatory network of ceRNA, we reveal a new mechanism of biliverdin in the treatment of CIR. The treatment of CIR provides new ideas.