心衰是临床常见的危重病，NT-proBNP是目前临床最为常用的心衰标志物，sST2则是近年来新发现的心衰标志物，可以有效评估心衰预后。现有检测方法以ELISA为主，操作繁琐，复杂费时。本项目建立了一种新型的时间分辨荧光免疫层析技术，此技术结合时间分辨荧光技术和免疫层析技术，具备两种技术的优点，快速灵敏，有效提高工作效率。利用包裹镧系元素Eu3+的纳米微球作为载体分别标记sST2抗体和NT-proBNP抗体，在免疫层析试纸条的两条检测带上分别包被sST2抗体和NT-proBNP抗体，滴加少量的血清，就可以在一个免疫层析试纸条上同时进行两种心衰标志物sST2和NT-proBNP的时间分辨荧光免疫定量检测。随后动态监测心衰动物模型及临床患者血清中的sST2和NT-proBNP,并与目前临床上使用的方法比对，研究其在心衰诊治和预后评估方面的应用。该技术为临床提供一种快速简便定量的新型检测技术。

Heart failure is a common critical disease in clinical. NT-proBNP is the most common used biomarkers of heart failure, while ST2 is a newly discovered biomarker of heart failure in recent years, which can effectively evaluate the prognosis of heart failure. Current detection method center on ELISA, that is operation complicated and time consumed. This project set up a novel time-resolved fluorescence immunochromatography technology, which combines time-resolved fluorescence immunoassay and chromatography technology. It has the advantages of both technologies, rapid and sensitive, and effectively improve the work efficiency. Using lanthanide Eu3+ labled nanometer microspheres to mark sST2 antibody and the NT - proBNP antibody, respectively. sST2 antibodies and the NT – proBNP antibody is respectively packaged in the immune chromatography test paper. We can quantitatively detect the two biomarkers in a immune chromatography test paper at the same time in time-resolved fluorescence immunoassay with a small amount of serum. Then, we dynamic monitoring sST2 and NT - proBNP in serum of patients and animal model of heart failure, and compared with the results detected by the currently used methods in clinical. Also we research its application in the diagnosis and treatment and prognosis evaluation of heart failure. The technique provides a quick and easy quantitative detection method for clinical.