我国大量的盐渍化土地限制了林农业发展和生态环境建设。外生菌根真菌可通过调控林木及其它植物体内离子平衡、组织渗透势以及水分吸收等方式来提高耐盐性；但是，相关的分子机制仍然不清楚。本研究拟利用杨树-双色蜡蘑菌根体系作为研究对象，在盐处理与对照下，开展杨树表型及生理特征测定和转录组分析，挖掘盐胁迫下菌根真菌和杨树各自产生的差异性表达基因，并阐述可能的相关性和相互作用关系；筛选其中差异表达最大2个来自真菌的关键基因，运用酵母双杂交、双分子荧光蛋白和免疫共沉淀等手段，阐明它们是否结合杨树根表达差异基因和如何调控杨树抗盐性。研究结果有助于理解外生菌根真菌提高树木及其它植物宿主抗盐性机理，同时也为利用微生物绿色提高林木抗盐性提供理论依据。

Soil salinity is one of the most severe abiotic stress factors that is causing increasing agricultural and environmental problems in China. Ectomycorrhizas can improve salt tolerance of trees and other plants through ion balance, osmotic adjustment and moisture absorption. However, little is known of the underlying molecular mechanism. In this project, we aim to use poplar-Laccaria bicolor ectomycorrhizal interaction as a model to investigate phenotypic and physiological changes, and the genes with contrasted expressions during salt stress by transcriptome analysis. We will clarify the origins (from ectomycorrhiza or poplar) and correlations between them. Then we will characterize molecular interaction of two such ectomycorrhiza genes with those from poplar and corresponding networks by yeast two hybrid, BiFC and CoIP. These results will not only elucidate molecular mechanism on how ectomycorrhizal fungi improve salt tolerance of poplars and other plants, but also provide a new method to improve abiotic stress of trees by using natural microbes.