转移是影响胃癌患者预后的最重要因素。研究表明长链非编码RNA(lncRNA)与胃癌转移密切相关，目前对lncRNA与胃癌转移的相关机制挖掘不足，更鲜有研究关注多个lncRNAs的协同影响。项目组首次从胃癌淋巴转移标本中筛选出两个lncRNA XLOC235和RP11-789，并证实二者对胃癌转移有重要影响，但其是否协同介导胃癌转移及具体分子机制尚不清楚。项目组发现二者均与smad4特定序列存在结合位点，提出两个lncRNA间存在缠绕绑定的“对话”机制，特异影响smad4转录并经Wnt信号通路协同介导胃癌转移的科学假设，拟利用体内外模型系统探讨XLOC235与RP11-789经smad4实现的“对话”作用模式和协同影响的信号通路变化对胃癌转移治疗的价值。此研究有利于阐明XLOC235和RP11-789协同影响胃癌转移的关键机制，为利用靶向lncRNA诊断和治疗胃癌转移提供更充分的科学依据。

Metastasis is the most important factor affecting the prognosis of patients with gastric cancer. Studies had shown that long non-coding RNAs(lncRNAs) were closely associated with metastasis of gastric cancer. However, the exploration of the molecular mechanism regarding the relation between lncRNAs and gastric cancer metastasis is insufficient, and there are few researches focusing on the synergistic effect of multiple lncRNAs. Our research group for the first time identified two lncRNAs, XLOC235 and RP11-789, from the metastasized lymph node specimens of gastric cancer. We further confirmed that they had important effects on gastric cancer metastasis. Yet whether these two lncRNAs could collaboratively mediate metastasis in gastric cancer and its specific molecular mechanisms remain unclear. We also found that both of these lncRNAs had binding sites to the specific sequences of smad4. On this basis, our group puts forward a hypothesis that there is a tangling "crosstalk" mechanism between lncRNA XLOC235 and RP11-789, which specifically influences transcription of smad4 and further mediates the invasion and metastasis of gastric cancer through WNT signaling pathway. In vitro and in vivo models will be used to systematically investigate the "crosstalk" mechanism of XLOC235 and RP11-789 , its synergistic effects to the WNT signaling pathways by smad4 and the significance for the treatment of gastric cancer metastasis. This study will help us understand the key mechanism of lncRNA XLOC235 and rp11-789 collaboratively influencing the metastasis of gastric cancer, providing a better scientific evidence of using lncRNAs as targeted therapy for the diagnosis and treatment for gastric cancer metastasis.