根叶互作诱导的细胞分裂素对多花黑麦草持续性再生的调控机制

以多花黑麦草组培克隆苗为试验材料，采用遮光生长、断根培养等方法，通过研究多次去叶调控的贮存碳水化合物对再生叶片生长素含量水平的影响，生长素由叶向根的传递，根系生长素对硝态氮吸收的影响，根系硝态氮吸收对叶片细胞分裂素含量水平的影响，以及叶片细胞分裂素对再生叶片生物量的影响，来揭示根叶互作诱导的细胞分裂素调控黑麦草持续性再生的机制。另外，通过研究多次去叶下根系硝态氮吸收所诱导的根系和叶片中的异戊烯基转移酶基因的表达，从基因表达的角度进一步深入揭示细胞分裂素对黑麦草持续性再生的调控机制，并通过研究持续性再生强弱不同的两个多花黑麦草品种，来明确与持续性再生密切相关的基因。

Tissue culture clone seedlings of italian ryegrass were used to study the continuous regrowth mechanism adjusted by cytokinin that was induced by the interactions of leave and root, with shading, roots breaking culture, and so on, through revealing the influence of stored carbohydrate induced by frequent defoliation on leaf auxin content, the auxin transmission from leaves to roots, the influence of root auxin on its nitrate absorbtion, the influence of nitrate absorbtion on leaf cytokinin content, and the corresponding effect of leaf cytokinin content on leaf biomass. Moreover, through studying isopentenyl transferase gene expression in leaves and roots induced by roots nitrate absorption under frequent defoliation, the continuous regrowth mechanism adjusted by cytokinins was revealed further in depth. Gene that is closely related to continuous regrowth was defined through studying isopentenyl transferees gene expression of two italian ryegrass cultivars with strong or weak continuous regrowth.