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丹参转录因子SmMYB50响应CMV侵染调控丹参有效成分生物合成的研究
结题报告
批准号:
82003890
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
范慧艳
依托单位:
学科分类:
中药资源
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
范慧艳
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中文摘要
黄瓜花叶病毒(CMV)是引起我国常用的大宗中药材丹参病毒病的主要病原物。感病植株根系细小,丹参酮等有效成分含量降低,严重影响了丹参的产量和品质。本研究前期工作发现,CMV侵染丹参可以诱导R2R3-MYB转录因子SmMYB50的特异性显著上调表达,且在丹参毛状根中过表达SmMYB50能够降低丹参酮的含量,提高丹酚酸的含量。推测SmMYB50可能是作用于丹参酮和丹酚酸合成代谢途径的转录因子,CMV通过影响SmMYB50的表达来参与丹参酮和丹酚酸的代谢调控。本项目拟以CMV和丹参为主要试验材料,深入研究SmMYB50对丹参酮/丹酚酸生物合成的转录调控机制,寻找靶标基因和转录因子结合位点,明确SmMYB50响应病毒侵染的生物学功能,并筛选与SmMYB50互作的参与抗病毒的寄主蛋白。此研究结果将揭示病毒在侵染过程中影响丹参有效成分积累的分子机制,为后续丹参品质的遗传改良提供科学依据。
英文摘要
Cucumber mosaic virus (CMV) is the causal agent of virus disease in Salvia miltiorrhiza Bunge,which is a commonly used as traditional Chinese medicine in our country. The roots of susceptible plants are always very thin and the contents of medicinal ingredient of tanshinones decreased greatly, which seriously affected the yield and quality of Salvia miltiorrhiza. Previously, we found that R2R3-MYB transcription factor SmMYB50 was specifically and remarkably up-regulated in response to CMV infection, and overexpression of SmMYB50 decreased tanshinones accumulation and increased the accumulation of phenolic acids in hairy roots of Salvia miltiorrhiza. Taken together, we propose that SmMYB50 might be a transcription repressor of tanshinones biosynthesis and a transcription activator of phenolic acids biosynthesis. CMV infection might be involved in metabolic regulation of tanshinones and salvianolic acids by regulating SmMYB50 expression. In this proposal, the transcriptional regulation mechanism of SmMYB50 on tanshinones/phenolic acids biosynthesis will be extensively investigated to find the target genes and transcription factor binding sites. Furthermore, the SmMYB50 biological function in response to virus infection will be researched and the antiviral host proteins interacting with SmMYB50 will be screened. These results will uncover molecular mechanisms of influencing the medicinal ingredients accumulation of Salvia miltiorrhiza in virus infection and provide scientific support for quality improvement breeding in Salvia miltiorrhiza.
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DOI:10.1094/pdis-11-20-2501-pdn
发表时间:2022
期刊:Plant Disease
影响因子:--
作者:Huiyan Fan;Tong Zhang;Jieqiong Sheng;Yang Zhou;Guoyin Kai
通讯作者:Guoyin Kai
尖孢镰刀菌侵染调控白术内酯生物合成的根 际微生态机制研究
  • 批准号:
    TGN24C140006
  • 项目类别:
    省市级项目
  • 资助金额:
    0.0万元
  • 批准年份:
    2024
  • 负责人:
    范慧艳
  • 依托单位:
国内基金
海外基金