萱草光周期途径核心因子CO调控二次开花花芽形成的分子机制研究

萱草是重要的商品花卉，二次开花特性是萱草的一个重要经济性状。项目前期研究结果表明光照长度对‘LC6’萱草当年分化型花芽的形成和秋季二次开花起着决定性的开关作用，因此萱草‘LC6’二次成花过程中花芽响应不同光长诱导的分子调控机制值得深入研究。本项目拟以具有重要种质资源价值的野生萱草‘LC6’（H. Lilioasphodelus sp. ‘LC6’）为主要研究对象，从“形态-环境-分子”3个层面，开展“萱草光周期途径核心因子CO调控二次开花花芽形成的分子机制”研究。通过研究不同光长诱导下花芽的成花-休眠进程差异，揭示‘LC6’萱草当年分化型花芽的形成规律。通过实时荧光定量PCR技术分析HlCO的表达模式特异性。通过拟南芥过表达载体构建技术进行HlCO功能验证。通过上游顺式作用元件、互作蛋白至下游调控基因的转录调控机制研究，揭示‘LC6’萱草当年分化型花芽在不同光周期诱导下成花与休眠转换过程。

Daylily is an important commodity flower, the secondary flowering characteristic is an important economic trait of daylilies. The preliminary research results of the project show that the length of light plays a decisive role in the formation of the differentiated flower buds of 'LC6' daylily in that year and the secondary flowering in autumn. The mechanism is worthy of in-depth study. This project intends to use the wild daylily 'LC6' （H. Lilioasphodelus sp. ‘LC6’）with important germplasm value as the main research object, from the "morphology-environment-molecule" three levels, to carry out "Hemerocallis photocycle pathway core factor CO regulation of secondary floral bud formation Mechanism " research. By studying the difference in flowering-dormancy process of flower buds induced by different light lengths, the formation rule of the differentiated flower buds of ‘LC6’ daylily was revealed. The specificity of HlCO expression pattern was analyzed by real-time fluorescence quantitative PCR technology. The HlCO function was verified by construction technology of Arabidopsis overexpression vector. Through the study of the transcriptional regulatory mechanism from upstream cis-acting elements and interacting proteins to downstream regulatory genes, finally reveals the molecular regulation mechanism of HlCO during the flowering and dormant transition of 'LC6' daylily floral bud under different photoperiods.