玉米SnRK1在籽粒代谢产物分配和生长发育中的调控网络解析

植物蔗糖非发酵-1相关蛋白激酶1（SnRK1）是维持碳、氮代谢平衡的重要调节因子，并在植物生长发育和胁迫响应中发挥重要作用。前期研究发现异源表达玉米SnRK1成员在调控拟南芥糖代谢、开花期和生物量中发挥了重要作用。本项目拟以ZmSnRK1.3基因过表达和敲除表达玉米株系为研究对象，通过高通量转录组测序技术进行基因差异表达谱分析，揭示其对下游基因的表达调控作用；通过代谢组学分析，明确其在玉米碳水化合物、蛋白质及脂类等代谢产物分配中的功能；同时，通过生化实验获得互作蛋白成员ZmOTU1，利用CRISPR/Cas9基因编辑技术和遗传学手段验证二者的上下游调控关系，从而解析ZmSnRK1.3调控代谢产物分配和植株生长发育的信号网络途径。该研究的开展对于玉米优异种质创新和基因工程育种具有重要意义。

Sucrose non-fermenting-1 (SNF1) -related protein kinase 1 (SnRK1) is a key regulator of carbon and nitrogen homeostasis, and plays critical roles in plant development and stress responses. Members of SNF1-related protein kinase 1 subfamily were isolated from maize (Zea mays L.) in our previous study and, results from heterologous expression of these members in Arabidopsis indicated that they play pivotal roles in sugar metabolism, flowering and plant biomass regulation. In this study, transgenic plants with ZmSnRK1.3 overexpressing or knockout will be used. Transcriptome analysis will be carried out to reveal the differential expression of downstream genes; Metabolomic analysis will be carried out to reveal the allocation of kernel metabolites, such as carbohydrate, protein and lipids; Partners that physically interact with ZmSnRK1.3 are screened and ZmOTU1 was obtained by biochemical methods. Based on CRISPR/Cas9 gene editing technology and genetic analysis, the regulatory relationship of ZmSnRK1.3 and ZmOTU1 will be revealed, and the signaling network of ZmSnRK1.3 in regulating kernel metabolism and plant development will finally be characterized. This study has important significance on maize germplasm innovation and genetically engineered breeding.