肠道细菌关键small RNAs在克罗恩病发生发展中的功能和作用机制

肠道菌群失调可导致炎症性肠病如克罗恩病（CD）的发生，深入研究肠道菌群与肠道黏膜之间的分子作用机制对CD发病机制的认识具有非常重要意义。本项目从肠道细菌sRNAs跨物种调控这一全新角度出发，研究在CD发生发展过程中的肠道细菌特征谱并发现一类CD特定的重要肠道细菌；研究CD特定肠道细菌的sRNA差异表达谱及其关键sRNAs的潜在靶点；阐明关键sRNAs对潜在靶点的分子调控机制及其在CD发生发展中的功能；探究肠道细菌关键sRNAs通过OMVs递送至宿主肠道细胞的作用机制。通过本项目研究，期望在阐明关键sRNAs跨物种调控克罗恩病的作用机制方面有所突破，为克罗恩病提供全新的可能干预靶点和治疗思路。

Intestinal flora is an important factor affecting the immune homeostasis of the intestinal tract. Intestinal flora imbalance can lead to the occurrence of inflammatory bowel disease such as Crohn's disease (CD). It is of great significance to study the molecular mechanism between intestinal flora and colonic mucosa. By studying the cross-species regulation of small RNAs (sRNAs) from intestinal bacteria, this project will identify the key intestinal bacteria in CD. The difference expression profiles of the key intestinal bacteria sRNAs will be established and the possible target genes that may be regulated by key sRNAs will be predicted. This project also aims to explain the way that key sRNAs from intestinal bacteria are delivered into colonic cells. The underlying molecular regulation mechanism and the functions of sRNAs in CD will be further clarified. The aim of this study is to clarify the biological function and regulation mechanism across species of key sRNAs from intestinal bacteria in CD, which will help to provide novel intervention targets and therapeutic approaches for CD treatment.