外泌体及其介导的信息传递是机体极其重要的免疫应答调控机制，也是当前生命科学研究的前沿热点，但是目前对海洋无脊椎动物外泌体及其免疫调控作用的研究极度匮乏。本项目在前期对灿烂弧菌刺激后的长牡蛎外泌体进行了转录组学和miRNA组学分析，并初步检测了其免疫调控作用。拟在此基础上，综合运用各种现代生物学技术手段，从蛋白质、mRNA和miRNA三种水平进一步发掘长牡蛎外泌体的组成成分，并明确这些成分对灿烂弧菌刺激的免疫应答特征；从转录和翻译两种水平测定长牡蛎外泌体对关键免疫信号转导通路的调控，从体液免疫和细胞免疫两个方面检测其对主要免疫指标的调节。综合上述结果，以组成成分为基础，以应答特征为桥梁，以调控机制为主线，初步阐释外泌体对长牡蛎抗灿烂弧菌免疫应答的调控机制。本项目的研究成果将为深入了解软体动物外泌体的免疫调控作用提供有力证据，并为海洋无脊椎动物固有免疫研究贡献新视角、新认知和新成果。

The exosomes have been confirmed to be involved in innate immune responses and play a pivotal role in the innate immunomodulation. However, the mechanism of immunomodulation mediated by exosomes in invertebrate innate immune system is still unclear. In our previous research, the exosomes isolated from oysters (Crassostrea gigas) stimulated by Vibrio splendidus or Staphylococcus aureus exhibited different feature of component, immune response profile and immunomodulation. Based on such achievements, the present research will be performed based on techniques from biochemistry, cell biology, molecular biology, molecular immunology and bioinformatics, aiming to (1) identified and analysis the component of oyster exosomes in protein, mRNA and microRNA (miRNA) level; (2) explore the immune response profile of these component after the stimulation of V. splendidus; (3) investigate the immunomodulation on immune signaling pathway mediated by exosomes in protein and mRNA level; (4) detect the humoral and cellular immune parameter changes in stimulation by exosomes from different source; (5) explore the immunomodulation mechanism of exosomes in oyster. The results of our research will reveal the mechanism of immunomodulation mediated by exosomes in mollusk and enhance the understanding of the immune defense system in invertebrates.