色泽发育是果实品质调控研究的重要内容。果树生产中普遍存在因着色不良导致的品质不佳等现象，开展果实色泽发育调控机理研究对提高果实品质至关重要。蓝莓果实富含花青素，具有极高的保健价值，但果实成熟过程中花青素积累调控的分子机制尚不清楚。本项目拟在课题组前期研究基础上，从组学水平研究NF-Y家族转录因子在蓝莓果实发育过程中的花青苷积累调控分子机制。项目将综合运用qRT-PCR、烟草脆裂病毒诱导的基因沉默技术、染色质免疫共沉淀、BiFC和Pull-Down及转基因等技术，结合串联组学分析、生理指标测定和显微观察等手段，筛选NF-Y转录因子的互作因子，鉴定其调控的下游靶基因，研究NF-Y转录因子在蓝莓果实成熟及花青苷积累调控中的作用，揭示果实成熟过程中信号交叉调控网络，阐明果实色泽发育与品质形成机理，为果树定向分子育种和优质高效栽培提供理论基础。

Color development is crucial to quality formation during fruit ripening. The phenomenon of poor quality resulted from poor coloring is widespread in fruit production. Therefore, the research on fruit color development and regulation mechanism is crucial. Blueberry fruit is rich in anthocyanin, which is of great benefit to human health, however, the mechanisms of anthocyanin accumulation in blueberry fruits during ripening are mostly unknown. In this project, we investigate the regulation of anthocyanin biosynthesis by the VcNF-Y transcriptional factor in blueberry fruits during ripening based on transcriptomic and proteomic changes. By the comprehensive use of qRT-PCR, VIGS, EMSA, BiFC, Pull-Down and transgenic technology, combined with integrating omics, physiological quantification, histological procedures and tissue localization, the functions of VcNF-Y transcriptional factor family in anthocyanin synthesis during blueberry fruit ripening will be elucidated on the basis of existing research, as well as its interacting factors and downstream target genes will be profiled. The results of the study will further clarify the intrinsic molecular mechanisms of fruit color and quality development, reveal the signal intersection and regulation network during fruit maturing, and ultimately provide theoretical basis for directed molecular breeding and cultivation of fruit tree.