CIRS-7调控miR-7a-5p/α-突触核蛋白通路介导的Parthanatos死亡对视网膜脱离感光细胞作用的基础与临床研究

视网膜脱离是一种常见且严重的致盲性眼病，感光细胞的死亡是影响患者术后视力恢复的主要原因。本课题组前期研究发现，视网膜脱离后存在感光细胞的坏死和凋亡，并伴有活性氧（ROS）和凋亡诱导因子（AIF）的升高。根据国际最新研究成果，ROS与AIF的升高与Parthanatos死亡有关，CIRS-7可以负向调控miR-7a-5p的表达，调节α-突触核蛋白的积聚，从而诱导细胞的PARP-1依赖的Parthanatos死亡。在预实验中我们也发现了视网膜脱离后PARP-1的高表达。所以本研究拟通过视网膜脱离模型的建立，应用PJ34、miR-7a-5p模拟物及CIRS-7干扰或过表达；并在临床上收集视网膜脱离患者的视网膜组织及玻璃体液，通过检测CIRS-7、miR-7a-5p和α-突触核蛋白等相关蛋白的表达，揭示Parthanatos死亡在视网膜脱离中的作用及其机制，为临床保护患者残存视功能提供依据。

Retinal detachment is a common and serious eye disease, and the death of photoreceptor cells is the main reason affecting the postoperative visual recovery of patients. Our previous study found that photoreceptors necroptosis and apoptosis existed after retinal detachment, accompanied by the increase of reactive oxygen species (ROS) and apoptosis-inducing factor (AIF). According to the latest international research, the increase of ROS and AIF are related to the Parthanatos, and CIRS-7 can regulates the expression of miR-7 and the accumulation of -synuclein, thus affecting the death of PARP-1 dependent Parthanatos . In the preliminary experiment, we also found the high expression of PARP-1 after retinal detachment. Therefore, this study intends to intervene with PJ34, miR-7a-5p mimics and AAV vector through the establishment of retinal detachment model and the retinal tissues、vitreous fluid of patients with retinal detachment were collected.The expression of pathway-related proteins ,CIRS-7, miR-7a-5p and α-synuclein were detected to provide a basic and clinical basis for the recovery of visual function of patients with retinal detachment.