Osa_miRNAs在水稻-稻曲病菌互作中的调控机制研究

随着水稻栽培模式及品种布局的改变，我国稻曲病逐年加重，已成为新的水稻三大病害之一。水稻对稻曲病菌的抗性资源有限，至今未发现主效抗性基因，利用传统的育种方法培育抗病品种短期内难以实现。越来越多的证据表明，植物与病原菌互作过程中，miRNAS起着重要调控作用。利用现代分子技术控制水稻抗病相关Osa_miRNAs的表达，可调控水稻抗病基因的表达从而增强水稻对病原菌的抗性，有望短期内获得抗病品种。本项目在前期筛选到抗性相关Osa_miRNAs的基础上，将通过5’-RLM-RACE分析及烟草共表达系统就Osa_miRNAs对预测靶标基因的调控进行验证，鉴定出参与水稻抗稻曲病的Osa_miRNAs。

Based on the large-scale growing of high-yielding hybrid cultivars and the change of rice farming system, rice false smut (RFS) has occurred severely in major rice-growing regions and become one of the top three rice diseases. Up to date, no major resistant gene has been detected, and breeding resistant cultivars by traditional methods cannot be realized in the short term. Increasing evidences showed that miRNAs play important roles in plant immunity against pathogens. Thus, it is possible to obtain resistant cultivars through regulating the expression of genes associated with resistance by controlling the expression of certain Osa_miRNAs. Recently, we made transcriptome analysis and screened out 3 Osa_miRNAs that showed different expression patterns in resistant and susceptible rice cultivars after inoculated with Villosiclava virens. In current study, the regulation of Osa_miRNAs on expression of the predicted targets will be analyzed by using 5’-RLM-RACE method and further evaluated in Nicotiana benthimiana.