细菌样颗粒（BLPs）是一种新型非遗传修饰型乳酸菌表面展示技术，外源蛋白可通过锚钩蛋白锚定于经热酸处理而得的乳酸菌肽聚糖骨架表面，形成空心抗原展示颗粒。通过激活宿主TLR2信号通路，BLPs作为黏膜疫苗在哺乳动物上显示出了安全、高效的独特优势。而家禽chTLR与哺乳动物不同，BLPs在家禽上是否具有同样的效应和机制尚不得而知。本研究拟以新城疫病毒（NDV）为家禽模式病原，构建同时展示囊膜糖蛋白F和HN的细菌样颗粒（NDV-BLPs），并重点探索家禽chTLR2亚类分子在NDV-BLPs诱导DCs细胞成熟以及直接激活淋巴细胞中的作用与机制，系统研究其通过激活chTLR2/MyD88信号通路增强家禽局部黏膜及系统免疫应答效应和方式，评价BLPs作为家禽黏膜疫苗构建平台的潜力。本研究将填补BLPs诱导家禽黏膜免疫应答机制的空白，为新城疫等家禽传染病新型黏膜疫苗和免疫调节剂的研发提供新的理论依据。

As an emerging technology for surface display on lactic acid bacteria without genetic modification, Bacterium-like particles (BLPs) have be used as an adjuvant and vaccine display system for mucosal delivery. Antigens are displayed on the particle surface as a fusion protein containing a peptidoglycan binding domain, called the protein anchor, that attaches non-covalently and with high avidity to the peptidoglycan wall, resulting in antigen display particles. As a novel development for mucosal vaccine, BLPs have shown a safe and efficient unique advantage in mammals by activating the host TLR2 signaling pathway. The TLRs of poultry are different from that of mammals. It is not sure whether BLPs have the same effect and mechanism in poultry as that in mammals. In this study, we will take Newcastle disease virus (NDV) as a model for poultry pathogens and construct the BLPs which display both the F and HN glycoproteins of NDV (NDV-BLPs). The main purpose of this study is to explore the action and mechanism of chTLR2 subtype molecules when NDV-BLPs induce maturation of dendritic cells and activate adaptive responses through direct interaction with lymphocytes. The systematic study on local mucosal and systemic immune responses of poultry induced by NDV-BLPs through activation of chTLR2/MyD88 signaling pathway will fill the blank of mucosal immunological mechanism involved BLP-based vaccines and display a broad space for development and application prospect of BLPs as mucosal vaccine delivery platform for poultry. This results will also provide a new scientific basis for development of mucosal vaccine or immune modulators for Newcastle disease and other infectious diseases in poultry.