重离子肿瘤治疗仍然存在“敏感性差异”问题，而决定重离子辐射敏感性的细胞分子水平参数并不完全清楚。本项目以不同射线敏感性肿瘤细胞为研究对象，以端粒功能障碍诱导的焦点(telomere dysfunction-induced foci，TIF)作为研究靶点，结合12C6+离子辐照，用免疫细胞化学和激光共聚焦显微扫描、流式细胞分析、蛋白以及基因表达等方法，探讨TIF表达的时间和剂量效应关系以及DNA损伤修复蛋白、shelterin复合物和端粒酶在TIF形成过程中的作用和机制，以证明TIF的表达差异对肿瘤细胞重离子敏感性的影响，及是否可以用TIF来预测肿瘤细胞重离子辐照敏感性。该研究有可能使TIF成为重离子治疗肿瘤潜在的分子靶点，为将重离子辐照用于治疗射线耐受性肿瘤提供新途径。

There is still the sensitivity difference problem in heavy-ion tumor therapy and the parameters of cell and molecular level determine heavy ion radiosensitivity is not entirely clear. In order to prove the effect of expression differences of telomere dysfunction induced foci (TIF) on heavy ion radiosensitivity and to determine whether TIF could be used to predict heavy ion radiosensitivity, in present study, tumor cells with different radiosensitivity are used as research object and TIF are used as research target, in combination with 12C6+ irradiation, using immunocytochemistry, confocal laser scanning microscopy, flow cytometry analysis, and protein and gene expression assay to explore the time and dose effect relationship of TIF expression and the function and mechanism of DNA damage and repair protein, shelterin complex and telomerase in the process of TIF formation. This study has the potential to make TIF as molecular targets for heavy ion therapy, and provide a new way in the treatment of radiation tolerance of tumor.