多囊卵巢综合征（PCOS）患者肠道菌群谱及药物治疗对肠道菌群结构的影响

多囊卵巢综合征（PCOS）是育龄期妇女中最常见的内分泌紊乱疾病，是女性生殖障碍疾病研究的重点，其病因复杂，发病机制不明确。PCOS与慢性炎症、胰岛素抵抗、代谢综合征等密切相关，这些代谢性疾病与肠道菌群谱的变化有着重要的关系。我们试图通过微生物测序技术，解析PCOS患者伴/不伴高雄激素血症和/或糖耐量异常情况下肠道菌群各自特点，绘制PCOS肠道菌群谱；研究对PCOS患者应用达英-35或二甲双胍治疗后，是否有助于调节患者肠道菌群结构，通过菌群移植明确肠道菌群与慢性炎症反应、胰岛素抵抗、高雄激素血症等的关联，从肠道菌群的角度阐明药物在改善高雄激素血症或糖耐量异常中的具体机制，明确PCOS发病及治疗有关的特异菌谱；随访妊娠结局，分析肠道菌群与妊娠结局是否关联，为PCOS患者肠道微生态平衡的重建提供数据，为开发以肠道菌群为靶向的药物或者益生菌提供基础，以指导临床对PCOS的诊断和用药，促进生殖健康。

Polycystic ovary syndrome (PCOS) is the most common endocrine disorders in women in childbearing age. As an important cause of infertility, it has always been the focus of female reproductive disorders researches. But its etiology is complex, and its pathogenesis is also unclear, and there is still no specific effective treatment by now. Since PCOS has close correlation with chronic inflammation, insulin resistance, and metabolic syndrome. And these metabolic diseases have important relationship with the profiles of gut microbiota, while in return, gut microbiota also affects the development of multiple endocrine and metabolic disorders. We try to use microbial sequencing technology, to analysis the changes of gut microbiota in PCOS patients with/without accompanied by hyperandrogenism and/or impaired glucose tolerance, and to draw the gut microbiota profiles of PCOS patients. We will also study whether diane-35 or metformin would help regulate the gut microbiota after the three month’s treatment. We will study its correlation with chronic inflammation, insulin resistance and hyperandrogenism, to clarify the mechanism of drugs during the treatment of hyperandrogenism and impaired glucose tolerance, and further acquire specific bacterial spectrum related to the development of PCOS. It will help providing theoretical basis for the development of target drugs on the gut microbiota and probiotics, which can guide the clinical diagnosis and treatment of PCOS more effectively, and help to guide the clinical diagnosis and medication of PCOS patients. So it can promote women’s reproductive health.