长链非编码RNA(LncRNA)作为一种非编码的调控性RNA分子来发挥作用已经被人熟知，本项目我们提出LncRNA通过编码短肽与转录因子互作从而调控肌肉生长的新作用模式。我们前期鉴定出由lncRNA-Six1编码的，一个7.2KD的短肽Small Six1，其与成肌细胞增殖分化有关，但是该短肽发挥作用的具体机制仍不清楚。本项目拟以lncRNA-Six1编码的短肽Small Six1结合MyoD为切入点，通过Co-IP等技术，对Small Six1结合MyoD的分子机理进行分析；结合蛋白质晶体学技术，解析Small Six1与MyoD蛋白复合物的晶体结构。本项目旨在明确短肽Small Six1结合MyoD的作用通路和分子调控网络，揭示lncRNA-Six1调控家鸡肌肉生长的新作用模式。本项目的完成对揭示动物肌肉生长的表观遗传机制有重要科学意义，同时对黄羽肉鸡生长性状的遗传改良有潜在应用价值。

It's well known that lncRNA could play a regulatory role by acting as a non-coding RNA molecule. In this study, we propose a new model that lncRNA regulates muscle growth, which is that lncRNA encodes a kind of short peptides that interact with transcription factors. In our previous study, we identified a 7.2KD short peptide (Small Six1) encoded by lncRNA-Six1, and found that this peptide is related to myoblast proliferation and differentiation. However, how the peptide Small Six1 regulates muscle growth remains unclear. In this study, we propose that the short peptide Small Six1 could interacts with MyoD. First, by using Co-IP technology, we will validate the interaction between Small Six1 encode by lncRNA-SIX1 and MyoD, and further to investigate the molecular mechanism of interaction. Then, we suppose to discover the crystal structure of Small Six1-MyoD protein complex using protein crystallography method. In this study, we aim to reveal the functional pathway and molecular regulatory networks of MyoD interacting by Small Six1, and further to reveal the new functional model that lncRNA-Six1 regulates muscle growth in chicken. The completion of this study is helpful with scientific significance for identifying of the epigenetic mechanism for muscle growth in animals, and is potentially valuable for the genetic improvement of growth traits for yellow chicken industry.