申请者在前一基金的研究中发现，长期集约经营显著降低毛竹林土壤碳储量及碳库稳定性。生物质炭输入被认为是增加土壤碳汇应对全球气候变化的重要途径之一；然而，其对亚热带人工林土壤碳过程的影响及其机理尚缺乏深入研究。本项目拟以毛竹人工林为研究对象，综合应用NMR、Py-GC-MS、FT-IR、元素分析、PLFA以及动态气室-IRGA法等分析技术，利用野外控制试验，研究竹叶生物质炭输入对不同集约经营历史毛竹林土壤碳储量、活性碳库、有机碳组分化学结构的影响；在此基础上，深入研究不同用量生物质炭输入及其与施肥措施协同影响下的土壤CO2通量、有机碳组分、活性碳库、微生物群落特征等因素的动态变化规律；再通过室内模拟培养试验，阐明生物质炭输入对毛竹林土壤有机碳矿化特征的影响及其对不同温度、湿度的响应规律；从而较系统地揭示生物质炭输入对毛竹林土壤碳汇功能的影响机理，为亚热带人工林土壤碳储存技术的研发提供科学依据。

Results of my previous NSFC funded project revealed that long-term intensive management significantly decreased soil carbon storage and stability in Moso bamboo (Phyllostachys pubescens) forests. Biochar addition is regarded as an important approach to enhance soil carbon sequestration and mitigate global climate change; however, its effects on soil carbon processes and the associated mechanisms in subtropical plantation soils are still unclear. Moso bamboo plantations will be selected as the study object in this project, and nuclear magnetic resonance (NMR), pyrolysis gas chromatography mass spectrometry (Py-GC-MS), Fourier-transform infrared spectroscopy (FT-IR), elemental analysis, phospholipid fatty acid (PLFA), and dynamic chamber-infrared gas analyzer (IRGA) methods will be adopted. Field experiments will be conducted to investigate the effects of biochar (made from bamboo leaf) addition on soil carbon storage, labile carbon pools, and chemical structure of organic carbon fractions. Furthermore, the dynamic changes in soil CO2 fluxes, organic carbon fractions, labile carbon pools, microbial community characteristic and other factors under different application rates of biochar and the synergistic effect of biochar addition and different fertilization practices will be intensively investigated. In addition, laboratory incubation experiments will be conducted to study the effects of different biochar addition rates on soil organic carbon mineralization in Moso bamboo forests under various soil temperature and moisture conditions. This project aims to elucidate the mechanisms associated with the impact of biochar addition on soil carbon sequestration in Moso bamboo plantations, and advance the development of technology for increasing carbon storage in subtropical plantation soils.