道路已成为最常见的廊道景观和人为干扰之一，对入侵植物的分布格局和传播扩散产生重要影响。交通路网的高速增长下，中国面临着路域生物入侵的巨大压力。道路沿线的生态调查揭示了入侵种当前的分布格局特征，但不能反映真实的入侵过程和定居历史。本研究以典型的道路廊道入侵植物紫茎泽兰(Ageratina adenophora)为研究对象，采用景观格局分析和分子遗传标记相结合的研究手段，以SSR标记分析其种群遗传结构和基因流，以基于二代测序技术的RAD标记分析其种群基因组结构，探讨道路景观对其遗传变异格局的作用机制，重建其入侵路线和种群扩散的历史过程，认识道路对其种群遗传多样性和基因流的影响，以及道路廊道和周围景观类型对其传播扩散的景观遗传效应。本研究将为认识入侵植物沿道路廊道传播扩散的机制提供新的视角，也为道路建设、管理和维护中防治生物入侵提供科学理论依据，丰富和发展景观遗传学和入侵生态学的研究。

Road is the most common corridor in the global human-dominated landscapes. It is also one of typical disturbance on the habitats. Road corridors play an important ecological role in the distribution and dispersal of nonindigenous plant invasion. Road network’s demands rapidly increase with the economy bloom in China, it has been producing great pressure on exotic invasiveness along the roads. Contemporary pattern of invasive plant populations established on road verges could be revealed by traditional ecological survey, but there were no solid information about its invading process and colonizing history. This proposal will conduct a research on one of the most noxious invasive alien plants in China, Ageratina adenophora, which is typically colonized and established along the roadsides. We will combine landscape pattern analysis with population genetic analysis by DNA markers. Population genetic background will be assessed by SSR markers, as long as population genome by restriction-site associated DNA sequencing (RAD-seq), in order to reconstruct its invading routes and population expansion in history. It will be fully probed that the spatial correlation between road corridor pattern and population genetic structure. We will put effort to understand how road corridors effect population genetic diversity and gene flow, and how road corridors facilitate population dispersal into adjacent landscape types and produce ecological and genetic consequences. We hope to cast a new insight on the mechanism of invasive plants along road corridors, and provide scientific comments and suggestion for preventing ecological invasion in road construction, management, and maintenance. The final results will enrich the study in landscape genetics and invasive ecology.