EGFR下游的K-ras基因被认为是启动癌变的关键，Hippo-YAP信号通路中YAP基因的过表达可促使肿瘤增殖及EMT过程，而YAP上游的转录共激活子TAZ受Akt/GSK-3β信号通路的调控。前期研究中，我们发现解毒三根汤（藤梨根、虎杖根和水杨梅根）通过EGFR/Hippo/YAP轴发挥抑制SW480细胞的迁移、侵袭以及逆转SW480细胞EMT的作用，但是Lats1沉默后，解毒三根汤仍然具有抗侵袭转移能力。所以我们猜测解毒三根汤可能通过Akt/GSK-3β信号通路作用干预TAZ进而抑制EMT的发生。本项目选用解毒三根汤应用体外结肠癌EMT模型，检测细胞侵袭转移能力及Hippo通路与Akt/GSK-3β通路蛋白、转移相关蛋白等磷酸化及表达水平，构建裸鼠结肠癌肝转移模型，分析Hippo信号通路与Akt/GSK-3β信号通路各基因的表达情况对结肠癌转移潜力的影响及解毒三根汤的作用。

K-ras gene downstream of EGFR is considered as the key to start the canceration and the overexpression of YAP gene in Hippo-YAP signal pathway may promote tumor proliferation as well as the process of EMT, while the transcriptional coactivator TAZ upstream of YAP is regulated by Akt / GSK-3β signal pathway. In the previous studies, we found that Jiedu Sangen Decoction (JSD, consisting of Polygonum cuspidatum, Geum japonicum thumnb, Radix actinidiae) plays an inhibitory effect in the migration, invasion of SW480 cells and reverses SW480 cells’ EMT by EGFR / Hippo / YAP axis. After Lats1 silenced, However, JSD still has the ability to inhabiting invasion and metastasis. It supposes that JSD intervenes TAZ by Akt / GSK-3β signal pathway thereby inhibiting EMT.. Using EMT vitro model of colon cancer intervened by JSD to detect the ability of cell invasion, metastasis and the expression levels of phosphorylated of Hippo singal pathway, Akt / GSK-3β signal pathway, metastasis-associated protein, etc. To construct colon cancer with liver metastasis model in nude mice, analyze the effect to the potential of colon cancer metastasis by the expression of each gene in Hippo singal pathway and Akt / GSK-3β signal pathway and the action of JSD, verify JSD’s inhibition of invasion and metastasis of colon cancer by regulating crosstalk between Hippo singal pathway and Akt / GSK-3β signal pathway.