加氧酶作为一种应答氧化损伤的关键蛋白，对机体发挥着重要的生物学作用。凡纳滨对虾是我国对虾养殖产业的最大优势品种，目前却尚未有关加氧酶的研究。本项目尝试从加氧酶的功能切入，深入研究加氧酶参与凡纳滨对虾免疫调控的机理。我们首先对加氧酶进行基因克隆及生物信息学分析，确定其蛋白表达、组织定位、生化活性和细胞学作用。继而分析凡纳滨对虾在各种不同环境胁迫下加氧酶基因、蛋白质表达谱及其活性。在此基础上我们利用RNA干扰技术探讨加氧酶对细胞形态、细胞凋亡和损伤、细胞免疫的调控作用及与其他免疫相关因子的关系。该研究旨在阐述加氧酶参与凡纳滨对虾免疫调控的崭新机理，为利用虾类自身的免疫调节基因加强抵抗外界环境胁迫的能力提供了可能途径。

As a key protein which can response to oxidative damage, oxygenase plays an important biological role on organism. Litopenaeus vannamei is the biggest variety of shrimp in the aquaculture industry of China, however, at present there is no oxygenase-related research on the shrimp. For the sake of discussing the mechanism of oxygenase involved in immunity regulation in the shrimp, this project makes an exhaustive study by the view of oxygenase function..Firstly we clone oxygenase from the shrimp, analyse its bioinformatics, confirm the protein expression, tissue localization, biochemical and cytological effect. Then we explore the transcription and translation regulation patter of oxygenase when the shrimp exposed to different environmental stress. In addition, by the application of RNA interference (RNAi) technology, we investigate the effect of oxygenase on cellular morphology, cell apoptosis and damage, regulation of cellular immunity and the relationship with other immune related factors. .This study aims to describe a new mechanism of how oxygenase participate in the immune regulation of Litopenaeus vannamei, which will provide a possible way to enhance the ability of resistance in shrimp under environmental stress.