基于肠道菌群调控DC-T细胞研究肺胃同治法治疗胃肠积热合并肺炎的作用机制

胃肠积热是无形热邪或有形热结壅于胃肠而致热盛的证态，与小儿肺炎发病相关。银莱汤以肺胃同治法组方，治疗小儿胃肠积热合并肺炎疗效确切，但其作用机制尚未明确。既往发现胃肠积热可引起肠道菌群紊乱，伴随肠黏膜局部免疫功能、机体免疫球蛋白与炎症因子水平改变，而银莱汤能够调节肠道菌群及机体免疫。研究表明，肠道菌群失调与肺炎发病相关,TLRs/NF-κB介导的DC-T细胞活化在菌群引起的免疫-炎症反应中发挥重要作用。基于此提出假说：胃肠积热引起肠道菌群失调，从而激活TLRs/NF-κB信号通路，活化DC细胞并调节T细胞分化，引起全身免疫功能失调，加重肺炎。本项目拟运用胃肠积热合并肺炎动物模型，以银莱汤进行干预，通过粪菌移植及体外的DC-T细胞共培养技术，明确菌群影响免疫功能的具体作用机制。本研究提出了小儿肺炎的新治法，并探讨其科学内涵，为该病的治疗提供了实验依据，对于提高该病的临床疗效具有现实意义。

Gastrointestinal heat retention syndrome is the syndrome of invisible heat or tangible heat knot in the gastrointestinal and heat retention which is closely related to children's pneumonia. Yinlai Tang was effective in the treatment of pneumonia by involving both lung and stomach, but the mechanism of its action is not clear. It has been found that gastrointestinal heat retention syndrome can cause intestinal flora disturbance, which is associated with the part immune function of intestinal mucosa and the changes of immunoglobulin and inflammatory factors. Yinlai Tang can regulate intestinal flora and immune function. Studies have shown that the imbalance of intestinal flora is related to the pathogenesis of pneumonia. TLRs/NF-κB mediating DC-T cell activation plays an important role in the immune inflammatory response caused by bacteria. Based on this, we put forward the hypothesis that gastrointestinal heat causes intestinal flora imbalance, thereby activating TLRs/NF-κB signaling pathway, activating DC cells and regulating T cell differentiation, causing systemic immune dysfunction, which aggravating pneumonia. Yinlai Tang would be used in this project treating pneumonia with gastrointestinal heat retention syndrome. Via fecal bacteria transplantation and DC-T cell co culture technique in vitro, this project aims to clarify the specific mechanism of the bacteria group affecting the immune function. This study puts forward a new treatment method for children pneumonia. By discussing its scientific connotation, this study would provide experimental evidence for the treatment of this disease and improve the clinical efficacy.