神经元是一种高度极化的细胞，主要由胞体，树突，轴突这三部分组成。神经元的极化是神经元发挥信息传递功能的基础。神经元极性的建立是一个复杂而精细的过程，在这过程中微管骨架的动态性是受到严格调控的，但这种调控具体是如何进行的人们了解的并不是很清楚。申请人在前期的研究工作中发现了一个对于神经元的极化起着关键作用的微管调节蛋白——Wdr47。本项目将在前期工作的基础上，以Wdr47基因敲除小鼠为实验模型,利用子宫内电转技术和体外神经元培养技术，深入研究Wdr47蛋白通过微管网络调控神经元极化的具体机制，进而为揭示神经元的极性建立过程提供重要的新知识。

Neurons are highly-polarized cells, which are constituted of cell bodies, dendrites and axons. The estabilishment of neuronal polarization is a complex and delicate process, in which the dynamics of microtubules is strictly regulated. However, the details of the regulation remain unclear.In our previous work, we uncovered Wdr47,a microtubule regulator,played vital roles in neuronal polarization. Based on our previous results obtained, we will use Wdr47 knockout mice as research models for this project, employing technologies of in-utero elecroporation and in-vitro neuron culture . We aim to elaborate the mechanism of how Wdr47 control neuronal polarization by affecting microtubule network,which will clarify the detailed process of neuron polarity establishment.